CAJ | 학술논문

近年来有关转基因产品可能带来的环境和食品安全问题的争论多集中在作为标记基因的抗生素抗性基因以及抗除草剂基因的广泛使用。因此寻找安全、有效的标记基因替代上述有争议标记基因就显得十分必要和紧迫。花青素合成酶类及其合成调控因子可以控制植物体内的色素合成，一些转化研究已证明其转化体表型发生了颜色改变。加之花青素类物质是一些天然色素，对人类有利而无害，可以利用花青素的这些特点，将花青素合成酶类及其调控因子基因作为一类可视化、安全和有效的标记基因来转化植物。本文就可视安全标记花青素合成酶类基因及其调控基因在植物转化研究中的应用进展进行了简要介绍，以期为提高标记基因的安全性提供参考。
근년래유관전기인산품가능대래적배경화식품안전문제적쟁론다집중재작위표기기인적항생소항성기인이급항제초제기인적엄범사용。인차심조안전、유효적표기기인체대상술유쟁의표기기인취현득십분필요화긴박。화청소합성매류급기합성조공인자가이공제식물체내적색소합성，일사전화연구이증명기전화체표형발생료안색개변。가지화청소류물질시일사천연색소，대인류유리이무해，가이이용화청소적저사특점，장화청소합성매류급기조공인자기인작위일류가시화、안전화유효적표기기인래전화식물。본문취가시안전표기화청소합성매류기인급기조공기인재식물전화연구중적응용진전진행료간요개소，이기위제고표기기인적안전성제공삼고。
Controversy on environment and food safety of transgenic products mainly focused on its containing antibiotics and herbicides-tolerant genes as selectable marker genes in recent years. Therefore, it is necessary and urgent to find safer and more efficient marker genes as alternatives. Some pigments, such as anthocyanin, synthesis in plants are controlled by anthocyanin synthesis enzymes and transcriptional regulatory factors. Some studies have demonstrated that visible color changes occurred in transformed phenotype by introducing these genes into plants. In addition, the products or intermediate products in anthocyanin biosynthesis pathway are all natural pigments which are benefitial to human health. Therefore, the genes or transcriptional regulatory factors responsible for anthocyanin biosynthesis could be used as selectable marker gene in plant transformation. In this paper, we gave a brief introduction of the research progress on plant transformation with visible marker genes, which was expected to provide a reference for selectable marker safety.