CAJ | 학술논문

为了提高脂肪酶在非水相中对手性仲醇的拆分效率，以2辛醇为模式底物，建立了以辛酸为酰基供体的无溶剂脂肪酶动力学拆分手性仲醇的反应体系，采用1?5∶1的酸醇摩尔比，45℃条件下反应12 h，2辛醇的转化率达到49?9％，并且S 2辛醇的对映体过量率e．e．s＝98?2％，反应的对映体比率E＞600。利用脂肪族仲醇与水形成共沸物的特性，通过非均相共沸蒸馏的方法提取拆分得到的手性仲醇，S 2辛醇的光学纯度并未降低，并且产率大于90％，产品纯度大于98％。
위료제고지방매재비수상중대수성중순적탁분효솔，이2신순위모식저물，건립료이신산위선기공체적무용제지방매동역학탁분수성중순적반응체계，채용1?5∶1적산순마이비，45℃조건하반응12 h，2신순적전화솔체도49?9％，병차S 2신순적대영체과량솔e．e．s＝98?2％，반응적대영체비솔E＞600。이용지방족중순여수형성공비물적특성，통과비균상공비증류적방법제취탁분득도적수성중순，S 2신순적광학순도병미강저，병차산솔대우90％，산품순도대우98％。
In order to enhance the efficiency of the kinetics resolution of secondary alcohols by lipase in non-aqueous phase, a solvent-free system was established using 2-octanol and octanoic acid as the model substrate and the acyl donor, respectively. When the reaction was carried out with n( acid)∶n( alcohol)=1?5∶1 at 45 ℃ for 12 h, the ( S)-2-octanol was obtained in e. e.=98?2% and the enantiomeric ratio reached E>600. The ability of aliphatic secondary alcohols to form an azeotrope with water was exploited to distill the enantiomerically pure secondary alcohol from the solvent-free system. The ee value of S-2-octanol was perfectly maintained. Furthermore, the yield and the purity of ( S)-2-octanol were more than 90% and 98%, respectively.