CAJ | 학술논문

目的：铜绿假单胞菌是医疗器械相关感染的主要致病菌，其持续感染的特性被认为与其具有形成附着生物膜的能力有关。本研究探讨硝酸镓涂膜对形成生物膜的抑制作用。方法将硝酸镓、明胶、EDTA按照一定的比例混合后涂于聚氯乙烯（ PVC）片表面，然后采用Kirby-Bauer法考察铜绿假单胞菌在PVC表面形成生物膜的情况并进行评估。结果表面涂有硝酸镓的PVC板在铜绿假单胞菌平板上形成的抑菌圈的直径大于20 mm，减少了细菌在表面的聚集并抑制了生物膜的形成。结论用硝酸镓涂膜对PVC表面进行前处理可抑制铜绿假单胞菌的生长和生物膜的形成。
목적：동록가단포균시의료기계상관감염적주요치병균，기지속감염적특성피인위여기구유형성부착생물막적능력유관。본연구탐토초산가도막대형성생물막적억제작용。방법장초산가、명효、EDTA안조일정적비례혼합후도우취록을희（ PVC）편표면，연후채용Kirby-Bauer법고찰동록가단포균재PVC표면형성생물막적정황병진행평고。결과표면도유초산가적PVC판재동록가단포균평판상형성적억균권적직경대우20 mm，감소료세균재표면적취집병억제료생물막적형성。결론용초산가도막대PVC표면진행전처리가억제동록가단포균적생장화생물막적형성。
Objective Pseudomonas aeruginosa is one of the main bacterial stain that cause device-associated infections . Pseudomonas aeruginosa may adhere to the surface of devices and form biofilm , which is responsible for the persistent infection .The present study was aimed to test the antimicrobial efficacy of gallium-coated PVC.Methods The antimicrobial efficacy of PVC segments coated through an instant dip method with gallium was determined .Using the modified Kirby-Bauer method ,gallium-coated PVC against Pseudomonas aeruginosa was evaluated .Results Gallium-coated PVC maintained a zone of inhibition of >20 mm against Pseudomonas aeruginosa for 1 week.Gallium-coated PVC significantly reduced the amount of viable Pseudomonas aeruginosa organisms adhering to their surfaces when compared with control .Conclusion Gallium-coated PVC impregnated using an instantaneous dip method , were shown to have prolonged antimicrobial durability and high efficacy in inhibiting adherence of Pseudomonas aeruginosa to PVC surface and formation of biofilm .