CAJ | 학술논문

目的：探讨人牙髓干细胞在体外成骨向分化潜能。方法：用免疫磁珠法分选人牙髓干细胞并检测其干细胞表面标志物Stro-1、 CD29、 CD34、 CD44、 CD45、 CD90、 CD105的表达。体外诱导人牙髓干细胞向成骨细胞分化，通过碱性磷酸酶染色和茜素红染色观察成骨诱导后细胞的成骨活性和矿化结节形成情况，通过real-time PCR分析成骨相关基因ALP、col I、RunX2、OC的表达，未成骨诱导细胞作为对照。结果：人牙髓干细胞阳性表达间充质干细胞表面标志物Stro-1、 CD29、 CD44、 CD90、 CD105，阴性表达造血干细胞表面标志物CD34、CD45。成骨诱导5d、7d、14d ALP染色阳性，21d茜素红染色仅诱导组可见明显钙结节形成，对照组为阴性。成骨相关基因ALP、RunX2和Col I mRNA在成骨诱导早期高表达，OC mRNA在成骨诱导14d表达开始逐渐升高。结论：经磁珠分选纯化的人牙髓干细胞在成骨诱导条件下可向成骨细胞分化，形成钙盐沉积和矿化结节， ALP、col I、RunX2、OC参与了成骨向分化的过程。
목적：탐토인아수간세포재체외성골향분화잠능。방법：용면역자주법분선인아수간세포병검측기간세포표면표지물Stro-1、 CD29、 CD34、 CD44、 CD45、 CD90、 CD105적표체。체외유도인아수간세포향성골세포분화，통과감성린산매염색화천소홍염색관찰성골유도후세포적성골활성화광화결절형성정황，통과real-time PCR분석성골상관기인ALP、col I、RunX2、OC적표체，미성골유도세포작위대조。결과：인아수간세포양성표체간충질간세포표면표지물Stro-1、 CD29、 CD44、 CD90、 CD105，음성표체조혈간세포표면표지물CD34、CD45。성골유도5d、7d、14d ALP염색양성，21d천소홍염색부유도조가견명현개결절형성，대조조위음성。성골상관기인ALP、RunX2화Col I mRNA재성골유도조기고표체，OC mRNA재성골유도14d표체개시축점승고。결론：경자주분선순화적인아수간세포재성골유도조건하가향성골세포분화，형성개염침적화광화결절， ALP、col I、RunX2、OC삼여료성골향분화적과정。
Objective:To investigate the capability of human dental pulp stem cells (hDPSCs) on osteogenic differentia-tion in vitro.Methods:hDPSCs were purified by magnetic-activated cell sorting with Stro-1 and the phenotypes were ana-lyzed with stem cell surface markers CD29, CD44, CD90, CD105, Stro-1, CD34, CD45. hDPSCs were treated continuous-ly with osteogenic inductive medium for 21. Alkaline phosphatase(ALP) staining, the expression levels of ALP, osteocalcin (OC), Collengen I(ColI), RunX2 genes and alizarin red staining for mineralization at different time points were analyzed. The non-induced cells were used as control.Results:Phenotype analysis indicated that hDPSCs were positive for mes-enchyme stem cell markers CD29, CD44, CD90, CD105, Stro-1, and negative for hematopoietic stem cell markers CD34, CD45. Compared with the control group, the ALP staining of the cells in induced group were significantly higher at day 5, 7, 14 and only the induced cells could form mineralized nodes as shown by alizarin red staining on Day 21. The expression of the ALP, ColI, RunX2, OC genes were positive in induced group.Conclusion:Human DPSCs selected by Stro-1 have the potential of differentiation into osteoblasts under osteogenic culture and forming mineralized nodes. Osteoblast markers (ALP, OC, ColI, RunX2 etc) participated in the osteogenic differentiation of hDPSCs.