CAJ | 학술논문

为了探寻连续降温对大菱鲆(Scophthalmus maximus)成鱼血清、肝脏及肌肉能量代谢等指标的影响,作者将大菱鲆从正常养殖水温18℃快速、连续降温至1℃,并在18℃、13℃、8℃、5℃、3℃和1℃共计6个温度点取血采样,分别测定血清中的总蛋白(TP)、白蛋白(ALB)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)、血糖(GLU)以及肝脏中的糖原和肌肉中的乳酸等指标。结果发现,随着温度降低,大菱鲆的血清TP浓度呈先下降后升高的趋势,各实验组与对照组无显著差异(P>0.05),但在13℃和3℃之间出现显著性差异(P<0.05)；血清 ALB 浓度则呈现升高趋势,在3℃时显著高于1℃(P<0.05)；血清TC浓度呈现升高趋势,且3℃时显著高于18℃(P<0.05)； GLU浓度呈先降低后升高趋势,且8℃、5℃、3℃实验组浓度显著高于其余实验组(P<0.05)；尿素浓度呈先降低后升高再降低趋势,且8℃时显著高于1℃(P<0.05)；肝糖原浓度呈先降低后升高趋势,且8、5、3℃实验组浓度显著高于其余实验组(P>0.05)；肌肉中乳酸含量呈先升高后降低趋势,且5℃时显著高于18℃(P<0.05)；其余指标则无显著性差异(P>0.05)。结果表明：当温度在18~8℃时,降温对大菱鲆体内代谢影响不大；当温度降到8~3℃时,大菱鲆体内开始出现应激反应；当温度继续降低至1℃时,大菱鲆体内的各种代谢基本处于停滞状态。综合分析各温度下的代谢指标得出结论,3~1℃温度区间与大菱鲆的生态冰温点比较接近,可以在此温度区间附近进一步进行无水保活的探索,在保证大菱鲆无水运输可行性的基础上优化经济成本。
위료탐심련속강온대대릉평(Scophthalmus maximus)성어혈청、간장급기육능량대사등지표적영향,작자장대릉평종정상양식수온18℃쾌속、련속강온지1℃,병재18℃、13℃、8℃、5℃、3℃화1℃공계6개온도점취혈채양,분별측정혈청중적총단백(TP)、백단백(ALB)、고밀도지단백담고순(HDL-C)、저밀도지단백담고순(LDL-C)、감유삼지(TG)、총담고순(TC)、혈당(GLU)이급간장중적당원화기육중적유산등지표。결과발현,수착온도강저,대릉평적혈청TP농도정선하강후승고적추세,각실험조여대조조무현저차이(P>0.05),단재13℃화3℃지간출현현저성차이(P<0.05)；혈청 ALB 농도칙정현승고추세,재3℃시현저고우1℃(P<0.05)；혈청TC농도정현승고추세,차3℃시현저고우18℃(P<0.05)； GLU농도정선강저후승고추세,차8℃、5℃、3℃실험조농도현저고우기여실험조(P<0.05)；뇨소농도정선강저후승고재강저추세,차8℃시현저고우1℃(P<0.05)；간당원농도정선강저후승고추세,차8、5、3℃실험조농도현저고우기여실험조(P>0.05)；기육중유산함량정선승고후강저추세,차5℃시현저고우18℃(P<0.05)；기여지표칙무현저성차이(P>0.05)。결과표명：당온도재18~8℃시,강온대대릉평체내대사영향불대；당온도강도8~3℃시,대릉평체내개시출현응격반응；당온도계속강저지1℃시,대릉평체내적각충대사기본처우정체상태。종합분석각온도하적대사지표득출결론,3~1℃온도구간여대릉평적생태빙온점비교접근,가이재차온도구간부근진일보진행무수보활적탐색,재보증대릉평무수운수가행성적기출상우화경제성본。
In order to study the effect of continuous cooling on metabolic indices of serum, liver and muscle, the adult turbot was subject to continuous cooling from 18 to 1℃, and 6 individuals were collected at each specific temperature including 18, 13, 8, 5, 3 and 1℃. The contents of liver glycogen, lactic acid of muscle and eight serum biochemical indices were measured including total protein, albumin, glucose, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglyceride and urea. The results showed that the total protein had significant difference between 13 and 3℃, albumin had significant difference between 3 and 1℃, total cholesterol had significant difference between 18 and 3℃, urea had significant difference between 8 and 1℃, contents of lactic acid in muscle had significant difference between 18 and 5℃.The contents of glucose in the 8, 5 and 1℃ groups were significantly higher than those in other groups. The contents of liver glycogen in the 8, 5 and 1℃groups were significantly higher than those in other groups. In addition, other indices showed no significant difference in the temperature change. The results showed that when the temperature ranged from 18 to 8℃, the metabolism changed quite little, when the temperature decreased from 8 to 3℃, the stress response appeared, when the temperature continuously decreased to 1℃, the metabolism tended to be extremely low, even stoped. According to the analysis of the data, a conclusion can be drawn that temperature between 3 and 1℃ was close to the ecological ice temperature of Scophthalmus maximus, so further exploration of anhydrous transport could be made in this temperature range to lessen the cost.