CAJ | 학술논문

【目的】解析半透明皱缩玉米胚乳形成机制．【方法】以纯合玉米突变体M-E-479为供试材料，通过对M-E-479籽粒进行表型鉴定、遗传分析、主要成分测定及扫描电镜观察，构建定位群体，利用图位克隆技术对突变基因进行遗传定位．【结果和结论】M-E-479突变体胚乳中脂肪含量增加，蛋白质和淀粉含量下降，淀粉粒的大小、形状、排列空间发生改变；用F2群体将突变基因定位在10号染色体SSR标记bnlg1074与umc1506之间，两标记的遗传距离约为3.6 cM，两标记的物理距离约为2.2 Mb．可见M-E-479是一个新的玉米半透明皱缩胚乳突变体，突变性状是由隐性单基因控制．
【목적】해석반투명추축옥미배유형성궤제．【방법】이순합옥미돌변체M-E-479위공시재료，통과대M-E-479자립진행표형감정、유전분석、주요성분측정급소묘전경관찰，구건정위군체，이용도위극륭기술대돌변기인진행유전정위．【결과화결론】M-E-479돌변체배유중지방함량증가，단백질화정분함량하강，정분립적대소、형상、배렬공간발생개변；용F2군체장돌변기인정위재10호염색체SSR표기bnlg1074여umc1506지간，량표기적유전거리약위3.6 cM，량표기적물리거리약위2.2 Mb．가견M-E-479시일개신적옥미반투명추축배유돌변체，돌변성상시유은성단기인공제．
Objective To analyze the formation mechanism of translucent and shrunken maize endo-sperm.[Method] The mutant M-E-479 of homozygous maize was used as experimental materials in our study .Through phenotypic identification , genetic analysis , main ingredients measurement and scanning electron microscopy , mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene .[Result and conclusion] The result showed that the amount of fat in-creased , while the protein and starch decreased , and the starch grains changed in size , shape and space configuration in the mutant endosperm of M-E-479 .The mutant gene is located between SSR markers bn-lg1074 and umc1506 of chromosome 10 by using F2 population .Meanwhile , the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb.The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant , and its mutant character may be controlled by a recessive single gene .