CAJ | 학술논문

【目的】建立一种用高效液相色谱法快速检测鸡肉组织中5种氟喹诺酮类药物（诺氟沙星、环丙沙星、达氟沙星、恩诺沙星、沙拉沙星）残留量的方法．【方法】样品的提取液由乙腈与三氯乙酸溶液（质量分数2％）按体积比1∶3的比例混合而成，流动相为乙腈-磷酸/三乙胺溶液（0.02 mol· L-1），荧光激发波长280 nm，发射波长450 nm．【结果和结论】添加低、中、高浓度水平时，回收率为84.71％～99.66％，变异系数为0.20％～4.34％．该方法检测诺氟沙星、环丙沙星和恩诺沙星的残留定量限为10μg · kg-1，达氟沙星的残留定量限为2μg · kg-1，沙拉沙星的残留定量限为20μg · kg-1．此法操作简单、实用，灵敏度高，可以满足鸡肉食品中氟喹诺酮类药物残留检测的需要．
【목적】건립일충용고효액상색보법쾌속검측계육조직중5충불규낙동류약물（낙불사성、배병사성、체불사성、은낙사성、사랍사성）잔류량적방법．【방법】양품적제취액유을정여삼록을산용액（질량분수2％）안체적비1∶3적비례혼합이성，류동상위을정-린산/삼을알용액（0.02 mol· L-1），형광격발파장280 nm，발사파장450 nm．【결과화결론】첨가저、중、고농도수평시，회수솔위84.71％～99.66％，변이계수위0.20％～4.34％．해방법검측낙불사성、배병사성화은낙사성적잔류정량한위10μg · kg-1，체불사성적잔류정량한위2μg · kg-1，사랍사성적잔류정량한위20μg · kg-1．차법조작간단、실용，령민도고，가이만족계육식품중불규낙동류약물잔류검측적수요．
Objective A method for rapid determination of five fluoroquinolones ( norfloxacin, ciprofloxa-cin, danofloxacin, enrofloxacin, sarafloxacin) residues in chicken muscle using high performance liquid chromatography ( HPLC) was established .[Method] The extracting solution of the chicken samples in-cluded a mixture of acetonitrile and trichloroacetic acid ( the mass fraction of 2%) .HPLC mobile phase consisted of acetonitrile and phosphate buffer (0.02 mol· L-1) with triethy lamine.Fluorometric excita-tion wavelength was 280 nm, and emission wavelength was 450 nm.[Result and conclusion] The recov-eries of 5 fluoroquinolones were 84.71%-99.66%at three spiked level(low, medium, high), and the relative standard deviations ( RSD) were 0.20%-4.34%.The limit of quantiation ( LOQ) of norfloxa-cin, ciprofloxacin and enrofloxacin was 10 μg· kg -1 .The LOQ of danofloxacin was 2 μg· kg -1 , and the sarafloxacin was 20μg· kg -1 .The method is simple , practical , and sensitive .It can meet the needs of determination of fluoroquinolones in chicken muscle .