福建农业学报
福建農業學報
복건농업학보
FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
2014年
5期
475-482
,共8页
林雄杰%范国成%胡菡青%王贤达%Yulu Xia%蔡子坚%刘波
林雄傑%範國成%鬍菡青%王賢達%Yulu Xia%蔡子堅%劉波
림웅걸%범국성%호함청%왕현체%Yulu Xia%채자견%류파
柑橘黄龙病%湿度%自然热罩%实时荧光定量PCR%影响
柑橘黃龍病%濕度%自然熱罩%實時熒光定量PCR%影響
감귤황룡병%습도%자연열조%실시형광정량PCR%영향
citrus huanglongbing%humidity%thermotherapy%real-time PCR%effect
比较加湿处理后热罩和自然条件下热罩对黄龙病的防治效果,结果表明自然条件处理后30 d叶片平均病菌浓度下降了85%以上,而加湿条件下叶片平均病菌浓度仅下降3.7%,与自然条件处理间差异达到显著水平;自然条件热罩处理后90 d叶片平均病菌浓度下降了97%以上,而加湿处理病树叶片平均病菌浓度下降了38%,与自然条件处理间的差异达到极显著水平。对照组叶片平均病菌浓度处理后30 d和90 d均有略微增加;加湿未热罩处理叶片平均病菌浓度在处理后30 d和90 d分别增加了4.5倍和8.7倍,但与对照组无显著差异。本研究结果表明加湿热罩处理病树显著减弱了其杀灭黄龙病菌效果,同时表明增加土壤湿度对黄龙病菌繁殖可能具有一定的促进作用。
比較加濕處理後熱罩和自然條件下熱罩對黃龍病的防治效果,結果錶明自然條件處理後30 d葉片平均病菌濃度下降瞭85%以上,而加濕條件下葉片平均病菌濃度僅下降3.7%,與自然條件處理間差異達到顯著水平;自然條件熱罩處理後90 d葉片平均病菌濃度下降瞭97%以上,而加濕處理病樹葉片平均病菌濃度下降瞭38%,與自然條件處理間的差異達到極顯著水平。對照組葉片平均病菌濃度處理後30 d和90 d均有略微增加;加濕未熱罩處理葉片平均病菌濃度在處理後30 d和90 d分彆增加瞭4.5倍和8.7倍,但與對照組無顯著差異。本研究結果錶明加濕熱罩處理病樹顯著減弱瞭其殺滅黃龍病菌效果,同時錶明增加土壤濕度對黃龍病菌繁殖可能具有一定的促進作用。
비교가습처리후열조화자연조건하열조대황룡병적방치효과,결과표명자연조건처리후30 d협편평균병균농도하강료85%이상,이가습조건하협편평균병균농도부하강3.7%,여자연조건처리간차이체도현저수평;자연조건열조처리후90 d협편평균병균농도하강료97%이상,이가습처리병수협편평균병균농도하강료38%,여자연조건처리간적차이체도겁현저수평。대조조협편평균병균농도처리후30 d화90 d균유략미증가;가습미열조처리협편평균병균농도재처리후30 d화90 d분별증가료4.5배화8.7배,단여대조조무현저차이。본연구결과표명가습열조처리병수현저감약료기살멸황룡병균효과,동시표명증가토양습도대황룡병균번식가능구유일정적촉진작용。
Citrus huanglongbing (HLB) is the most destructive disease of citrus ,and there is no effective control agent at the present ,but control citrus psyllid ,excavation of diseased trees .In this paper ,we analyzed the effection of humidity on Citrus Huanglongbing thermotherapy in the field .Our results showed that 30 d nature and moist thermotherapy decrease the average concentration of Las in the leaves by 85% and 3.7% .Furthermore ,90 d nature and moist thermotherapy decrease the average concentration of Las in the leaves by 97% and 38% .In the contrast , the average concentrations of Las in leaves under control were slightly increased after 30 d and 90 d treatments ;30 d and 90 d humidification without sheeting increased the average concentration of Las in the leaves by 4.5-fold and 8.7-fold ,but there was no significant difference with the control .Our results suggest that humidification with sheeting decrease the effect on killing Las significantly and increasing the soil moisture will benefit for the breeding of Las .