CAJ | 학술논문

为解决细叶石仙桃人工栽培种苗短缺问题，以细叶石仙桃蒴果为材料，应用植物组织培养技术，筛选不同阶段所需的最适培养基。结果表明其种子可在MS＋20 g·L -1蔗糖＋8 g·L -1琼脂培养基上萌发，发芽率可达70％以上；MS ＋50g·L -1土豆＋20g·L -1蔗糖＋8g·L -1琼脂培养基有利于原球茎分化小苗，低浓度6-BA和NAA组合适合原球茎增殖与芽的分化；在1/2MS ＋0.5 mg·L -1 NAA＋20 g·L -1蔗糖＋8 g·L -1琼脂培养基上生根率达100％，通过以上系列培养基的培养可获得完整植株。
위해결세협석선도인공재배충묘단결문제，이세협석선도삭과위재료，응용식물조직배양기술，사선불동계단소수적최괄배양기。결과표명기충자가재MS＋20 g·L -1자당＋8 g·L -1경지배양기상맹발，발아솔가체70％이상；MS ＋50g·L -1토두＋20g·L -1자당＋8g·L -1경지배양기유리우원구경분화소묘，저농도6-BA화NAA조합괄합원구경증식여아적분화；재1/2MS ＋0.5 mg·L -1 NAA＋20 g·L -1자당＋8 g·L -1경지배양기상생근솔체100％，통과이상계렬배양기적배양가획득완정식주。
To alleviate a seed shortage on Pholidota cantonensis Rolfe ,artificial culturing with medium optimization was investigated by using the plant tissue culture technology .The results showed that MS+20 g · L -1 sucrose+8 g · L -1 agar was an appropriate medium that allowed a germination rate above 70% .MS+50 g · L -1 potato+20 g · L -1 sucrose+8 g · L -1 agar was desirable for the plantlet′s protocorm differentiation .Low concentrations of 6-BA and NAA in medium was better for bud protocorm proliferation and differentiation .The medium formulated with 1/2 MS+0.5 mg · L -1 NAA+20 g · L -1 sucrose+ 8 g · L -1 agar was optimal for rooting ,as a rate of 100%was achieved .By using the combination of the above-mentioned media at respective stages ,the plantlets of P . cantonensis Rolfe could be rapidly obtained from seeds .