CAJ | 학술논문

利用16个亲本及特异种质92gk729的39个衍生品系为材料，采用221对SSR标记和系谱追踪等位变异法分析祖先亲本在籼粳杂交各衍生世代的遗传贡献。结果表明：（1）在92gk729中有10对SSR引物位点上出现4个祖先亲本杂交重组的特异条带，有5个重组后的特异位点能稳定传递到衍生后代中，有1个重组后的特异位点在亲本及衍生品系中都没有出现；（2） ketan Nangka、嘉南粳、02428、明恢72对92gk729的遗传贡献率分别为15.09％、21.32％、22.78％和34.64％。4个亲本杂交染色体片段重组后能产生新的特异位点，其遗传贡献率是6.17％；（3）4个祖先亲本在每条染色体上对衍生后代遗传贡献的趋势都是随着衍生代数的递增，遗传贡献率逐渐下降。亲本对各世代衍生品系的遗传贡献率为明恢72＞02428＞嘉南粳＞Kenta Nangka ；（4）采用系谱追踪等位变异法进行数据分析，然后对数据进行聚类，发现同一杂交组合的品系、同一系谱分支存在衍生关系的大部分品系聚为一类，后代亲缘关系相近的高代材料聚为一类。说明该法能够较好地反映祖先亲本对后代的遗传贡献。
이용16개친본급특이충질92gk729적39개연생품계위재료，채용221대SSR표기화계보추종등위변이법분석조선친본재선갱잡교각연생세대적유전공헌。결과표명：（1）재92gk729중유10대SSR인물위점상출현4개조선친본잡교중조적특이조대，유5개중조후적특이위점능은정전체도연생후대중，유1개중조후적특이위점재친본급연생품계중도몰유출현；（2） ketan Nangka、가남갱、02428、명회72대92gk729적유전공헌솔분별위15.09％、21.32％、22.78％화34.64％。4개친본잡교염색체편단중조후능산생신적특이위점，기유전공헌솔시6.17％；（3）4개조선친본재매조염색체상대연생후대유전공헌적추세도시수착연생대수적체증，유전공헌솔축점하강。친본대각세대연생품계적유전공헌솔위명회72＞02428＞가남갱＞Kenta Nangka ；（4）채용계보추종등위변이법진행수거분석，연후대수거진행취류，발현동일잡교조합적품계、동일계보분지존재연생관계적대부분품계취위일류，후대친연관계상근적고대재료취위일류。설명해법능구교호지반영조선친본대후대적유전공헌。
In order to analyze the genetic contribution of the ancestors to the offsprings from rice subspecies cross , 16 parents and 39 varieties derived from special germplasm (92gk729) were analyzed by 221 pairs of SSR primers and pedigree allele method .The results showed that :(1) In 92gk729 ,the special fragment of the recombination of the four ancestors were amplified with 10 pairs of SSR primers ;5 specific sites of the recombination could stably transmit into the offsprings ;one specific site of the recombination did not appear at the ancestors and the offsprings , so these special fragments need to be further analyzed . (2 ) The genetic contribution ratio of Ketan Nangka , JiaNanJing ,02428 and Minghui72 to 92gk729 was 15.09% ,21.32% ,22.78% and 34.64% , respectively .The hybridization and recombination of the chromosome segment of the four parents amplified new specific sites ,whose genetic contribution rate was 6.17% .(3) On every chromosome ,the trend of the genetic contribution from the four ancestors to the offsprings was gradually decreased ,accompanied with the increase of generations .The genetic contribution from the four ancestors to the offsprings showed Minghui72>02428>JiaNanJing>Kenta Nangka .(4) The cluster analysis of the data gained by the pedigree allele method was performed .The results showed that of the same hybrid combination ,most of the varieties of the same genealogical branch with the derivative relations could be clustered together ;the material of high derivative generations in close sibship of descendant could be clustered together .We could conclude that pedigree allele method could better reflect the genetic contribution of the ancestors to the offsprings .