CAJ | 학술논문

以马大杂种相思组培苗为研究对象，通过筛选最佳的增殖培养基，调整增殖时间，探讨芽体长度、继代次数对增殖的影响，增殖培养对生根的影响，建立马大杂种相思腋芽高效组培增殖体系。结果表明：将健壮的初代培养无菌芽剪成含1个腋芽的茎段，接入最佳增殖培养基(改良 MS +6-BA1.5 mg·L -1+NAA0.1 mg·L -1+Ac0.04 g·L -1，添加蔗糖3%和琼脂7 g·L -1)中连续培养7代，7次继代的平均增殖倍数为2.52，增殖时间为35天，单株平均繁殖系数为14.99，且7次继代增殖倍数变化小、增殖芽质量好。将增殖芽的茎段(含2～3个腋芽)接入1/2MS+IBA1.0 mg·L -1+ NAA0.5 mg·L -1(添加蔗糖3%和琼脂7 g·L -1)进行生根培养，15天生根率可达96.5%，移栽后存活率为95.0%。
이마대잡충상사조배묘위연구대상，통과사선최가적증식배양기，조정증식시간，탐토아체장도、계대차수대증식적영향，증식배양대생근적영향，건립마대잡충상사액아고효조배증식체계。결과표명：장건장적초대배양무균아전성함1개액아적경단，접입최가증식배양기(개량 MS +6-BA1.5 mg·L -1+NAA0.1 mg·L -1+Ac0.04 g·L -1，첨가자당3%화경지7 g·L -1)중련속배양7대，7차계대적평균증식배수위2.52，증식시간위35천，단주평균번식계수위14.99，차7차계대증식배수변화소、증식아질량호。장증식아적경단(함2～3개액아)접입1/2MS+IBA1.0 mg·L -1+ NAA0.5 mg·L -1(첨가자당3%화경지7 g·L -1)진행생근배양，15천생근솔가체96.5%，이재후존활솔위95.0%。
To build an efficient axillary bud multiplication system of Acacia mangium × A. auriculiformis,the aseptic plantlets of A. mangium × A. auriculiformis were used to screen the optimal protocol for in vitro multiplication. The optimal multiplication medium and period,length of the sterile stems,subculture times,rooting and transplant survival rate were studied. The results showed that after being cultured 7 times on improved-MS+ 0. 5 mg·L -16-BA+0. 1 mg·L -1 NAA +0.04 g·L -1Ac(with combination of 3% sugar and 7 g·L -1 agar),which was the best multiplication medium screened from the trial test,the average proliferation times of the stem with one axillary bud were 2. 52,and the propagation coefficient of the plantlet was around 14. 99 after 35 days. Moreover,the plantlets grew well and there was no obvious variation of the multiplication ratio. The aseptic stem with 2 or 3 axillary buds was inoculated to 1/2MS +IBA1. 0 mg·L -1 +NAA0. 5 mg·L -1 ( with combination of 3% sugar and 7 g·L -1 agar) for rooting culture,the rooting rate was 96. 5% in 15 day and the transplant survival rate was 95. 0%.