CAJ | 학술논문

为了明确浙江省红枝鸡爪槭枝枯病的病原种类，在浙江省四明山区域采集红枝鸡爪槭枝枯病样品，以组织分离法进行病原物的分离培养，对分离得到的炭疽病菌落进行纯化和单菌丝段分离后，以形态学为基础，参照 Sutton分类系统进行鉴定。结果表明：从病样中共分离到6个炭疽病菌菌株。菌株的分生孢子圆柱形，单孢无色，具1个油球，两端钝圆，大小(18.23～11.76)μm ×(4.46～2.94)μm(平均14.79μm ×4.06μm)；附着孢淡褐色，近圆形或不规则形，边缘平滑，大小为8.67μm ×4.52μm，与胶孢炭疽病菌相似。按照柯赫氏法则对红枝鸡爪槭植株上的当年生枝进行致病性测定，证实胶孢炭疽菌对红枝鸡爪槭的致病性。将菌株 Z92011的 rDNA-ITS序列与 GenBank 中相关菌株的ITS序列进行同源性比较，与胶孢炭疽病菌[GenBank登录号 KF787111]的同源性达到100%。确定浙江省红枝鸡爪槭枝枯病的病原菌为胶孢炭疽病菌。试验表明用40%咪鲜胺乳油800倍液喷雾防治效果达92.6%。
위료명학절강성홍지계조축지고병적병원충류，재절강성사명산구역채집홍지계조축지고병양품，이조직분리법진행병원물적분리배양，대분리득도적탄저병균락진행순화화단균사단분리후，이형태학위기출，삼조 Sutton분류계통진행감정。결과표명：종병양중공분리도6개탄저병균균주。균주적분생포자원주형，단포무색，구1개유구，량단둔원，대소(18.23～11.76)μm ×(4.46～2.94)μm(평균14.79μm ×4.06μm)；부착포담갈색，근원형혹불규칙형，변연평활，대소위8.67μm ×4.52μm，여효포탄저병균상사。안조가혁씨법칙대홍지계조축식주상적당년생지진행치병성측정，증실효포탄저균대홍지계조축적치병성。장균주 Z92011적 rDNA-ITS서렬여 GenBank 중상관균주적ITS서렬진행동원성비교，여효포탄저병균[GenBank등록호 KF787111]적동원성체도100%。학정절강성홍지계조축지고병적병원균위효포탄저병균。시험표명용40%미선알유유800배액분무방치효과체92.6%。
To explore the pathogens of branch blight of Acer palmatum‘Sangokaku’in Zhejiang province,The infected samples were collected from Siming mountain area of Zhejiang in September 2013 . The pathogens were isolated by tissue isolation method from blighted A. palmatum ‘Sangokaku ’ branch and cultured on standard medium. The obtained colonies were identified as Colletotrichum according to the taxonomic system of Sutton BC after being subcultured and single hyphal fragment. The results showed that the six isolates were obtained from the sampling sites. Their conidia were cylindrical,colorless single spore,with one oil ball,blunt ends,ranged from (18. 23 -11. 76) μm × (4. 46 -2. 94)μm ( average 14. 79 μm × 4. 06 μ m) . The appressoria were nearly round or irregular in shape,edge smoothing,pale brown,average 8. 67 μm × 4. 52 μ m. Conidia and appressoria were similar with Colletotrichum gloeosporioides in shape and size. Pathogenicity tests were performed by strain Z920121. After inoculation,the same branch blight was observed in 23 days on 100% of inoculated branches. DNA was directly extracted from the mycelium of Z920121 grown on PDA for 10 days. The identities of the isolates were confirmed by ITS1-5. 8S-ITS2 rDNA sequence ( GenBank Accession No. KF787111 ) analysis that showed 100% sequence similarity to C. gloeosporioides. ( Accession No. KC493156. 1, HQ645082. 1 ) . It was identified that the pathogen of branch blight of A. palmatum‘Sangokaku’in Zhejiang province was C. gloeosporioides. Treatment by spraying the plant with 40% EC 800-times diluted prochloraz liquid had a control effect on the disease up to 92. 6%.