CAJ | 학술논문

测定我国东北松嫩沙地和呼伦贝尔沙地自然生境中不同科属7种沙生植物高温时段叶抗氧化酶活力、渗透调节物和丙二醛(MDA)含量的日变化。结果表明：1)7种沙生植物所测定指标的日均最高和最低值，叶丙二醛含量相差2～3倍；超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活力分别相差2.2，7.5，2.5倍；脯氨酸、可溶性蛋白质、可溶性糖含量分别相差35，1.0，2.0倍。2)猪毛菜和差不嘎蒿日均 MDA 含量较低，日均 SOD，POD，CAT 活力和脯氨酸、可溶性蛋白质和糖含量也较低；扁蓿豆和小叶锦鸡儿日均 MDA 含量较高，日均POD和 CAT活力、脯氨酸、可溶性蛋白含量也较高；叶细胞膜脂过氧化程度与抗氧化酶活力和渗透调节含量正相关。3)在渗透调节物种类上，豆科植物依靠脯氨酸，黄柳和冰草依靠可溶性糖；在抗氧化酶种类上，扁蓿豆、小叶锦鸡儿和冰草依靠 POD和 CAT，胡枝子和黄柳依靠 SOD。可见，东北沙地7种植物通过维持高抗氧化酶活力和渗透调节物含量抑制膜脂过氧化以适应风沙环境。
측정아국동북송눈사지화호륜패이사지자연생경중불동과속7충사생식물고온시단협항양화매활력、삼투조절물화병이철(MDA)함량적일변화。결과표명：1)7충사생식물소측정지표적일균최고화최저치，협병이철함량상차2～3배；초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)활력분별상차2.2，7.5，2.5배；포안산、가용성단백질、가용성당함량분별상차35，1.0，2.0배。2)저모채화차불알호일균 MDA 함량교저，일균 SOD，POD，CAT 활력화포안산、가용성단백질화당함량야교저；편숙두화소협금계인일균 MDA 함량교고，일균POD화 CAT활력、포안산、가용성단백함량야교고；협세포막지과양화정도여항양화매활력화삼투조절함량정상관。3)재삼투조절물충류상，두과식물의고포안산，황류화빙초의고가용성당；재항양화매충류상，편숙두、소협금계인화빙초의고 POD화 CAT，호지자화황류의고 SOD。가견，동북사지7충식물통과유지고항양화매활력화삼투조절물함량억제막지과양화이괄응풍사배경。
In this study,the daily changes of antioxidant enzyme activities,the contents of malondialdehyde ( MDA) and osmoregulation substances were measured in leaves of Salix gordejevii,Artemisia halodendron,Melissitus ruthenicus, Salsola collina,Agropyron cristatum,Caragana microphylla,and Lespedeza bicolor in different family and genera grown respectively in natural habitats of Hulunbuir desert and Songnen desert. The results showed 1 ) Among 7 species of psammophytes,the maximum value of average daily MDA content was higher 2 -3 times than the minimum one; the maximum value of average daily activities of superoxide dismutase ( SOD ) ,peroxidase ( POD ) ,catalase ( CAT ) was respectively higher 2 . 2 ,7 . 5 and 2 . 5 times than the minimum one; the maximum value of average daily contents of proline,soluble protein and soluble sugar was respectively 35 times,1 times and 2 times than the minimum one. 2) S. collina and A. halodendron had not only lower average daily content of MDA,but also lower average daily activities of SOD,POD,and CAT,and lower average daily contents of proline,soluble protein and soluble sugar. While,M. ruthenicus and C. microphylla had higher average daily content of MDA and activities of POD and CAT,and higher average daily contents of proline,soluble protein,indicating that the degree of membrane lipid peroxidation was positively correlated with antioxidant enzyme activity and osmoregulation substance contents. 3 ) In terms of kinds of osmotic adjustment substances,leguminous plants depended on proline,while S. gordejevii and A. cristatum depended on soluble sugar. In terms of kinds of antioxidant enzymes,M. ruthenicus,C. microphylla and A. cristatum hinged on CAT and POD; while,S. gordejevii and L. bicolor required for SOD. This study suggests that 7 species of psammophytes would keep higher activities of antioxidant enzymes and contents of osmotic adjustment substances to inhibit lipid peroxidation and maintain cell oxygen free radical metabolism balance to adapt to desert environment.