CAJ | 학술논문

研究海南淮山茎腐病菌株对戊唑醇的敏感性，以期为该病害的综合防治提供实践和理论依据。通过单孢分离获得32株茎腐病菌，分别采用菌丝生长速率法和孢子萌发法测定戊唑醇对各菌株的毒力，并进行毒力回归和敏感性分析。结果表明：戊唑醇对菌丝生长具有较强的抑制作用，其抑制中质量浓度（EC50）最小值、最大值、平均值分别为0.063797、0.407005、（0.2283±0.0805）μg/m L ；该药对孢子萌发的抑制效果更明显，其 EC50最小值、最大值、平均值分别为0.002550、0.104858、（0.0318±0.0242）μg/m L。正态分析表明：菌丝生长对戊唑醇的敏感性呈正态分布；而孢子萌发的敏感性呈非正态分布，其最大EC50和最小EC50之间的跨度较大，EC50表现不连续，在高质量浓度区仍有不敏感菌株存在，这表明在自然界中存在个别抗药性较强的淮山茎腐病菌株。
연구해남회산경부병균주대무서순적민감성，이기위해병해적종합방치제공실천화이론의거。통과단포분리획득32주경부병균，분별채용균사생장속솔법화포자맹발법측정무서순대각균주적독력，병진행독력회귀화민감성분석。결과표명：무서순대균사생장구유교강적억제작용，기억제중질량농도（EC50）최소치、최대치、평균치분별위0.063797、0.407005、（0.2283±0.0805）μg/m L ；해약대포자맹발적억제효과경명현，기 EC50최소치、최대치、평균치분별위0.002550、0.104858、（0.0318±0.0242）μg/m L。정태분석표명：균사생장대무서순적민감성정정태분포；이포자맹발적민감성정비정태분포，기최대EC50화최소EC50지간적과도교대，EC50표현불련속，재고질량농도구잉유불민감균주존재，저표명재자연계중존재개별항약성교강적회산경부병균주。
In order to provide practical and theoretical basis for the prevention and control of Dioscorea op posita stem rot disease ,the bioassay method of baseline sensitivity was developed on the basis of the effect of tebuconazole on the mycelium grow th and spore germination of Fusarium oxysporum f .sp .dioscorea .Thirty-two strains were obtained by single spore isolation .Virulence of tebuconazole on all of the strains was tested by the methods of mycelium grow th and spore germination .Then virulence regression equation was constructed and the sensitive distribution was analysed .The results showed that mycelium growth of the pathogen was affected by tebuconazole ,with the maximal EC50 of 0.407 005 μg/mL ,minimum EC50 of 0.063 797 μg/mL , and mean EC50 of (0.228 3 ± 0.008 5)μg/mL ,respectively .And spore germination was inhibited by tebuconazole to a greater extent ,with the mean EC50 as (0.031 8 ± 0.024 2)μg/mL ,ranging from 0.002 550 μg/mL to 0.104 858 μg/mL .Normal distribution analysis showed that the sensitivity of mycelium growth to tebuconazole fitted well with a normal distribution function ,but that of spore germination did not ,for which the span between the maximal EC50 and the minimum EC50 was big .In high concentration area ,there were still insensitive strains .This may be related to the change of environment ,and thus a few strains possessed a certain resistance .