河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2014年
6期
30-34
,共5页
文雁成%鲁丽萍%张书芬%王建平%朱家成%何俊平%赵磊%曹金华
文雁成%魯麗萍%張書芬%王建平%硃傢成%何俊平%趙磊%曹金華
문안성%로려평%장서분%왕건평%주가성%하준평%조뢰%조금화
十字花科%油菜%种间杂交%亲和指数%琴形叶%高油酸%低亚麻酸
十字花科%油菜%種間雜交%親和指數%琴形葉%高油痠%低亞痳痠
십자화과%유채%충간잡교%친화지수%금형협%고유산%저아마산
Cruciferae%rape%interspecific hybridization%compatibility index%fiddle-shaped leaf%high olein acid content%low linolenic acid content
为了拓宽甘蓝型油菜种质资源的遗传基础,以甘蓝型油菜、白菜型油菜和芥菜型油菜为材料研究了十字花科种间杂交的可行性。结果表明,不同物种间油菜杂交结角率和亲和指数存在差异。在芥菜型油菜与甘蓝型油菜、白菜型油菜种间杂交中,芥菜型油菜作母本的杂交结角率(分别为95.0%、92.5%)和亲和指数(分别为6.51、12.40)高于其反交(17.5%、20.0%,0.56、1.12)。在甘蓝型油菜与白菜型油菜的种间杂交中,以甘蓝型油菜为母本的杂交结角率和亲和指数(分别为97.5%和14.66)高于以白菜型油菜作母本(分别为30.0%和2.41)。将芥菜型油菜的琴形叶性状转移到甘蓝型油菜中,发现该性状由1对隐性基因控制。育成了油酸含量高于80%的特高油酸含量新品系和亚麻酸含量低于3%(2.14%)的甘蓝型油菜品系,这些优异脂肪酸种质资源材料均是低芥酸、低硫甙品系,可以直接应用于生产。
為瞭拓寬甘藍型油菜種質資源的遺傳基礎,以甘藍型油菜、白菜型油菜和芥菜型油菜為材料研究瞭十字花科種間雜交的可行性。結果錶明,不同物種間油菜雜交結角率和親和指數存在差異。在芥菜型油菜與甘藍型油菜、白菜型油菜種間雜交中,芥菜型油菜作母本的雜交結角率(分彆為95.0%、92.5%)和親和指數(分彆為6.51、12.40)高于其反交(17.5%、20.0%,0.56、1.12)。在甘藍型油菜與白菜型油菜的種間雜交中,以甘藍型油菜為母本的雜交結角率和親和指數(分彆為97.5%和14.66)高于以白菜型油菜作母本(分彆為30.0%和2.41)。將芥菜型油菜的琴形葉性狀轉移到甘藍型油菜中,髮現該性狀由1對隱性基因控製。育成瞭油痠含量高于80%的特高油痠含量新品繫和亞痳痠含量低于3%(2.14%)的甘藍型油菜品繫,這些優異脂肪痠種質資源材料均是低芥痠、低硫甙品繫,可以直接應用于生產。
위료탁관감람형유채충질자원적유전기출,이감람형유채、백채형유채화개채형유채위재료연구료십자화과충간잡교적가행성。결과표명,불동물충간유채잡교결각솔화친화지수존재차이。재개채형유채여감람형유채、백채형유채충간잡교중,개채형유채작모본적잡교결각솔(분별위95.0%、92.5%)화친화지수(분별위6.51、12.40)고우기반교(17.5%、20.0%,0.56、1.12)。재감람형유채여백채형유채적충간잡교중,이감람형유채위모본적잡교결각솔화친화지수(분별위97.5%화14.66)고우이백채형유채작모본(분별위30.0%화2.41)。장개채형유채적금형협성상전이도감람형유채중,발현해성상유1대은성기인공제。육성료유산함량고우80%적특고유산함량신품계화아마산함량저우3%(2.14%)적감람형유채품계,저사우이지방산충질자원재료균시저개산、저류대품계,가이직접응용우생산。
In order to broaden the genetic basis of germplasm in Brassica napus ,the feasibility of creating novel germplasm by cruciferous interspecific hybridization was investigated in this article .Results implied that podding ratio and crossing compatibility index differed among different cruciferous species .In both interspecifichybridizationsof BrassicanapusandBrassicajuncea,BrassicarapaandBrassicajuncea,the podding ratios (95.0% and 92.5% ) and crossing compatibility indexes (6.51 and 12.40 ) w ere much higher when Brassica juncea was used as maternal parent than those in reciprocal crosses ,which were 17.5% and 20.0% ,0.56 and 1.12 .In the interspecific hybridization of Brassica napus and Brassica rapa ,the podding ratio(97.5% ) and crossing compatibility index (14.66) when Brassica napus was used as maternal parent were much higher than those of its reciprocal cross ,which were 30.0% and 2.41 , respectively .By interspecific hybridization between Brassica juncea and Brassica napus ,the character of fiddle-shaped leaf in Brassica juncea was transferred to Brassica napus ,which was controlled by one pair of recessive gene .In this study ,Brassica napus lines with high olein acid content (83.44% ) and low linolenic acid content (2.14% ) were obtained .These novel germplasms also had low erucic acid and glucosinolate content ,and could be directly used in production .