CAJ | 학술논문

为了建立野生稻的脱分化与分化体系，以普通野生稻为试验材料，通过在N6D、NB基本培养基中添加不同质量浓度激素（IAA、NAA、2，4 D、6 BA、KT ），统计野生稻在不同诱导培养基上的出愈率，从而确定最佳诱导培养基，并观察胚性愈伤组织分化成苗的情况。结果发现，以N6D为基本培养基，添加质量浓度分别为6.0 mg/L、3.0 mg/L、3.0 mg/L、1.5 mg/L 的2，4 D、NAA、IAA、KT ，接种7 d后出愈率高达56.7％，愈伤组织直径为0.31 cm ，将其转入MS分化培养基，24 d后分化成苗。说明该诱导培养基是培养普通野生稻产生胚性愈伤组织的最佳培养基。
위료건립야생도적탈분화여분화체계，이보통야생도위시험재료，통과재N6D、NB기본배양기중첨가불동질량농도격소（IAA、NAA、2，4 D、6 BA、KT ），통계야생도재불동유도배양기상적출유솔，종이학정최가유도배양기，병관찰배성유상조직분화성묘적정황。결과발현，이N6D위기본배양기，첨가질량농도분별위6.0 mg/L、3.0 mg/L、3.0 mg/L、1.5 mg/L 적2，4 D、NAA、IAA、KT ，접충7 d후출유솔고체56.7％，유상조직직경위0.31 cm ，장기전입MS분화배양기，24 d후분화성묘。설명해유도배양기시배양보통야생도산생배성유상조직적최가배양기。
The N6D and NB medium containing different hormones (IAA ,NAA ,2 ,4 D ,6 BA and KT)were used to study the callus rate of Oryza rufipogon Griff ,so as to determine the best medium for O .ruf ipogon Griff .And the shoot induction rate from embryonic calli was also ob-served .The result showed that ,after the seeds were placed on medium for 7d ,the callus ratio from N6D medium with 6.0 mg/L 2 ,4 D ,3.0 mg/L NAA ,3.0 mg/L IAA ,1.5 mg/L KT was 56.7% ,while the diameter of callus reached to 0.31 cm .And then the callus of O .ruf ipogon Griff were transferred to MS dedifferentiation medium for 24 d ,the callus became the green plants .This is a quick system for differentiation and dedifferentiation of O .ruf ipogon Griff .