CAJ | 학술논문

建立了猪肉、猪肝和猪尿中阿托品残留的超高效液相色谱-串联质谱（ UP LC-MS/MS ）的检测方法。样品经乙腈-0．1％甲酸水（ V/V，90∶10）溶液提取，Agela Cleanert? PEP-2固相萃取柱净化后，采用UPLC-MS/MS测定，外标法定量。结果表明，阿托品在0．2～10 ng/mL范围内线性关系良好，相关系数大于0．99。在0．5～2．5μg/kg 添加浓度范围内阿托品的批间平均回收率78．3％～98．2％，批内、批间相对标准偏差均小于15％，检测限为0．2μg/kg，定量限为0．5μg/kg。该方法简便、灵敏度高、重现性好，适用于猪肉、猪肝及猪尿中阿托品含量的测定。
건립료저육、저간화저뇨중아탁품잔류적초고효액상색보-천련질보（ UP LC-MS/MS ）적검측방법。양품경을정-0．1％갑산수（ V/V，90∶10）용액제취，Agela Cleanert? PEP-2고상췌취주정화후，채용UPLC-MS/MS측정，외표법정량。결과표명，아탁품재0．2～10 ng/mL범위내선성관계량호，상관계수대우0．99。재0．5～2．5μg/kg 첨가농도범위내아탁품적비간평균회수솔78．3％～98．2％，비내、비간상대표준편차균소우15％，검측한위0．2μg/kg，정량한위0．5μg/kg。해방법간편、령민도고、중현성호，괄용우저육、저간급저뇨중아탁품함량적측정。
A method based on ultra performance liquid chromatography coupled with tandem mass spectrometry ( UPLC-MS/MS) was developed for determination of atropine in porcine muscle,liver and urine. The analyte was extracted with acetonitrile-0.1%formic acid in water ( V/V,90 ∶ 10) ,and was cleaned up by the Agela Cleanert? PEP-2cartridge. Quantification of the analyte was achieved by UPLC-MS/MS with multiple reaction monitoring ( MRM) using external standard. The results indicated that the correlations were greater than 0.99 at the range of 0.2~10 ng/mL. The average recoveries of the drug ranged from 78.3%~98.2%at spiked levels of 0.5~2.5μg/kg with RSD less than 15%. The detection limit and quantification limit was 0.2μg/kg and 0.5μg/kg, respectively. The method was convenient, sensitive and reproducible for monitoring atropine residues in porcine muscle,liver and urine.