浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
7期
881-884
,共4页
变应性鼻炎%消风宣窍汤%IgE%IL-4%IFN-γ%组胺
變應性鼻炎%消風宣纖湯%IgE%IL-4%IFN-γ%組胺
변응성비염%소풍선규탕%IgE%IL-4%IFN-γ%조알
al ergic rhinitis%Xiaofeng Xuanqiao decoction%IgE%IL-4%IFN-γ%histamin
[目的]探讨消风宣窍汤治疗变应性鼻炎（Al ergic Rhinitis，AR）的作用机理。[方法]将60只Hartley豚鼠随机分为正常组、模型组、消风宣窍汤高、中、低剂量组、氯雷他定组。卵蛋白(OVA)建立变应性鼻炎豚鼠模型，药物干预后，ELISA法检测各组豚鼠血清总IgE、IL-4、IFN-γ、组胺的水平。[结果]与正常组比较，模型组IgE、IL-4、组胺水平显著升高、IFN-γ水平明显下降(P<0.01)；与模型组比较，消风宣窍汤高剂量组能显著降低血清总IgE、IL-4、组胺水平，升高IFN-γ水平（P<0.01），消风宣窍汤中、低剂量组、氯雷他定组能降低血清总IgE、IL-4、组胺水平，升高IFN-γ水平（P<0.05）。[结论]消风宣窍汤能促进IFN-γ的产生，抑制IL-4的形成，促进Th1/Th2分泌的细胞因子之间恢复平衡，从而减轻EOS的活化，减少IgE的合成，减少组胺的释放，从多方面阻止变应性鼻炎的炎症反应，达到治疗效果。
[目的]探討消風宣纖湯治療變應性鼻炎（Al ergic Rhinitis，AR）的作用機理。[方法]將60隻Hartley豚鼠隨機分為正常組、模型組、消風宣纖湯高、中、低劑量組、氯雷他定組。卵蛋白(OVA)建立變應性鼻炎豚鼠模型，藥物榦預後，ELISA法檢測各組豚鼠血清總IgE、IL-4、IFN-γ、組胺的水平。[結果]與正常組比較，模型組IgE、IL-4、組胺水平顯著升高、IFN-γ水平明顯下降(P<0.01)；與模型組比較，消風宣纖湯高劑量組能顯著降低血清總IgE、IL-4、組胺水平，升高IFN-γ水平（P<0.01），消風宣纖湯中、低劑量組、氯雷他定組能降低血清總IgE、IL-4、組胺水平，升高IFN-γ水平（P<0.05）。[結論]消風宣纖湯能促進IFN-γ的產生，抑製IL-4的形成，促進Th1/Th2分泌的細胞因子之間恢複平衡，從而減輕EOS的活化，減少IgE的閤成，減少組胺的釋放，從多方麵阻止變應性鼻炎的炎癥反應，達到治療效果。
[목적]탐토소풍선규탕치료변응성비염（Al ergic Rhinitis，AR）적작용궤리。[방법]장60지Hartley돈서수궤분위정상조、모형조、소풍선규탕고、중、저제량조、록뢰타정조。란단백(OVA)건립변응성비염돈서모형，약물간예후，ELISA법검측각조돈서혈청총IgE、IL-4、IFN-γ、조알적수평。[결과]여정상조비교，모형조IgE、IL-4、조알수평현저승고、IFN-γ수평명현하강(P<0.01)；여모형조비교，소풍선규탕고제량조능현저강저혈청총IgE、IL-4、조알수평，승고IFN-γ수평（P<0.01），소풍선규탕중、저제량조、록뢰타정조능강저혈청총IgE、IL-4、조알수평，승고IFN-γ수평（P<0.05）。[결론]소풍선규탕능촉진IFN-γ적산생，억제IL-4적형성，촉진Th1/Th2분비적세포인자지간회복평형，종이감경EOS적활화，감소IgE적합성，감소조알적석방，종다방면조지변응성비염적염증반응，체도치료효과。
Objective Discuss the treatment mechanism of Xiaofeng Xuanqiao Decoction on guinea pig with al ergic rhinitis. [Methods]Sixty guinea pigs were randomized into 6 groups: normal control group, al ergic rhinitis(AR) group, high-dosage, equivalent-dosage, low-dosage Xiaofeng Xuanqiao decoction group, and loratadine group. AR guinea pig model was successful y established by ovalbumin(OVA) chal enge method. Xiaofeng Xuanqiao decoction and loratadine were applied to intervene AR guinea pig. The serum levels of IgE and IL-4, IFN-γ, histamine were determined by ELISA methods. [Results]The expression levels of IgE and IL-4, histamine significantly increased,meanwhile the level of IFN-γsignificantly lower compared with the normal group(P<0.01). Compared with that in AR model group, the serum level of IgE and IL-4, histamine in high-dosage Xiaofeng Xuanqiao decoction group significantly decreased,but the level of IFN-γ increased(P<0.01); the levels of IgE and IL-4, histamine decreased in other medication groups, meanwhile the level of IFN-γ increased compared with the model group(P<0.05).[Conclusion]Xiaofeng Xuanqiao can promote the produce of IFN-γ, inhibit the formation of IL-4, promote the secretion of Th1/Th2 cytokines to restore balance, to relieve eosinophil activation, reduce the synthet-ic of IgE, reduce the release of histamine, in many ways to prevent al ergic rhinitis inflammation, to achieve therapeutic effect.
