CAJ | 학술논문

目的：探讨不同质量囊胚活检后的继续发育潜力和玻璃化冻融后的复苏能力。方法选择体外受精-胚胎移植周期患者的剩余胚胎，根据授精后第5/6天囊胚质量等级分为高质量囊胚组和低质量囊胚组，两组囊胚根据活检与否分别分为活检组和对照组。其中活检组囊胚采用激光切割结合抽吸法活检滋养层细胞，而后将活检成功囊胚进行玻璃化冷冻；对照组囊胚不活检，直接进行玻璃化冷冻。比较不同分组囊胚的活检结局及玻璃化冻融效果。结果低质量囊胚活检成功率低于高质量囊胚，但差异无统计学意义（P＝0.183），低质量囊胚活检后玻璃化冻融复苏率显著低于高质量囊胚（ P＝0.001），但低质量囊胚和高质量囊胚活检组的玻璃化冻融复苏率与对照组相比差异均无统计学意义（ P＝0.597，P＝0.823）。结论囊胚期活检不影响胚胎继续发育能力及玻璃化冻融复苏效果。
목적：탐토불동질량낭배활검후적계속발육잠력화파리화동융후적복소능력。방법선택체외수정-배태이식주기환자적잉여배태，근거수정후제5/6천낭배질량등급분위고질량낭배조화저질량낭배조，량조낭배근거활검여부분별분위활검조화대조조。기중활검조낭배채용격광절할결합추흡법활검자양층세포，이후장활검성공낭배진행파리화냉동；대조조낭배불활검，직접진행파리화냉동。비교불동분조낭배적활검결국급파리화동융효과。결과저질량낭배활검성공솔저우고질량낭배，단차이무통계학의의（P＝0.183），저질량낭배활검후파리화동융복소솔현저저우고질량낭배（ P＝0.001），단저질량낭배화고질량낭배활검조적파리화동융복소솔여대조조상비차이균무통계학의의（ P＝0.597，P＝0.823）。결론낭배기활검불영향배태계속발육능력급파리화동융복소효과。
Objective To investigate the impact of blastocyst biopsy on the development potential and sur-vival rate after vitrification of different quality blastocysts .Methods The surplus embryos in IVF-ET cycles were al-located to good or poor quality blastocysts group by the embryo grade in day 5/6, and the same quality blastocysts were allocated to biopsy and control group .For the blastocysts from biopsy group trophectoderm removal was per-formed and then the survival blastocysts were vitrificated , but for control group vitrification was performed without bi-opsy.The survival rate after blastocyst biopsy and warming were compared between different groups .Results For the poor quality blastocysts group the survival rate after blastocyst biopsy was lower than the good quality blastocysts group, but the difference was not significant ( P=0.183 ) , and the survival rate after warming was significantly lower than the good quality blastocysts group ( P=0.001 ) .But the survival rate after warming was similar between the biop-sy group and control group(P=0.597,P=0.823).Conclusion Blastocyst biopsy did not impair the development potential and survival efficient after vitrification .