CAJ | 학술논문

目的：了解河南省风疹病毒分离株的基因特征，为制定风疹的预防和控制策略提供依据。方法分离病毒，RT-PCR获取风疹病毒E1基因扩增产物，核苷酸序列测定，进行E1基因核苷酸与氨基酸特征分析。结果河南省风疹病毒分离株以1 E基因型为主，1 F基因型仅在2002年出现，2 B基因型自2013年开始出现；E1基因核苷酸序列同源性为87．8％～100％，氨基酸序列同源性为67．0％～100％，血凝抑制位点和中和位点等重要抗原位点均未发生变化，抗原性稳定，1 E基因型的第338位氨基酸发生Leu到Phe的改变。结论河南省风疹病毒分离株以1 E基因型为主。
목적：료해하남성풍진병독분리주적기인특정，위제정풍진적예방화공제책략제공의거。방법분리병독，RT-PCR획취풍진병독E1기인확증산물，핵감산서렬측정，진행E1기인핵감산여안기산특정분석。결과하남성풍진병독분리주이1 E기인형위주，1 F기인형부재2002년출현，2 B기인형자2013년개시출현；E1기인핵감산서렬동원성위87．8％～100％，안기산서렬동원성위67．0％～100％，혈응억제위점화중화위점등중요항원위점균미발생변화，항원성은정，1 E기인형적제338위안기산발생Leu도Phe적개변。결론하남성풍진병독분리주이1 E기인형위주。
Objective To investigate the genetic characteristics of E 1 gene of rubella virus strains isolated in Henan province for further investigation on rubella prevention and control .Methods RNA was extracted from rubella virus strains isolated from suspected measles cases in Henan during 2008 to 2012 .E1 gene of the isolates were amplified by RT-PCR and sequenced .The nucleotide ( nt ) and amino acid ( aa ) sequences of E1 gene of Henan isolates were aligned with the sequences of other reference strains downloaded from GenBank.Phylogenetic tree was constructed by using MEGA 6.0 software package.Results The pre-dominant genotype of rubella virus isolated in Henan was 1E genotype.No 1F genotype was detected.The 2B genotype emerged in 2013.The E1 gene of Henan isolates shared 87.8%-100% homologies in nucleo-tide sequences and 67.0%-100%in amino acids sequences .No variation was detected at hemagglutination inhibition and neutralization sites .A Leu to Phe mutation occurred at amino acid site 338 of 1E genotype in all isolates.Conclusion The results of this study demonstrated that genotype 1E was the predominant geno-type of rubella virus epidemic in Henan province .