CAJ | 학술논문

目的：考察七叶皂苷对大鼠肝微粒体的蛋白含量、CYP450酶总量和主要CYP450酶亚型（CYP1A2、CYP2D6、CYP3A4和CYP2C19）活性的影响。方法分别以0.9％NaCl和苯巴比妥作为空白组和阳性对照组，并以乙醇-5％葡萄糖溶液作为溶剂对照组，大鼠灌胃给予七叶皂苷（4.0 mg· kg-1· d-1），连续7 d，测定各组肝微粒体蛋白含量、CYP450蛋白含量以及CYP1A2、CYP2D6、CYP3A4及CYP2C19活性，并进行比较。结果与溶剂对照组相比，七叶皂苷给药组大鼠肝微粒体蛋白含量略增加，但差异无统计学意义（ P ＞0.05）；肝微粒体CYP450含量无明显差异。七叶皂苷给药后，CYP1A2和CYP3A4活性增加，但无统计学意义（P＞0.05）；CYP2D6和CYP2C19的活性没有改变。结论七叶皂苷对大鼠肝微粒体蛋白浓度、CYP450酶总量和CYP450酶亚型的活性均无影响。
목적：고찰칠협조감대대서간미립체적단백함량、CYP450매총량화주요CYP450매아형（CYP1A2、CYP2D6、CYP3A4화CYP2C19）활성적영향。방법분별이0.9％NaCl화분파비타작위공백조화양성대조조，병이을순-5％포도당용액작위용제대조조，대서관위급여칠협조감（4.0 mg· kg-1· d-1），련속7 d，측정각조간미립체단백함량、CYP450단백함량이급CYP1A2、CYP2D6、CYP3A4급CYP2C19활성，병진행비교。결과여용제대조조상비，칠협조감급약조대서간미립체단백함량략증가，단차이무통계학의의（ P ＞0.05）；간미립체CYP450함량무명현차이。칠협조감급약후，CYP1A2화CYP3A4활성증가，단무통계학의의（P＞0.05）；CYP2D6화CYP2C19적활성몰유개변。결론칠협조감대대서간미립체단백농도、CYP450매총량화CYP450매아형적활성균무영향。
Objective To study the effect of Aescine on the content of livermicrosome protein , cytochrome P450 ( CYP450 ) and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in cytomicrosome of rat liver.Methods Rats were given Aescine by oral administration ( 4.0 mg· kg-1 · d-1 ) for 7 days to detect the content of livermicrosome pro-tein and CYP450 and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in cytomicrosome taking 0.9%NaCl, ethanol-5%glucose so-lution and phenobarbital as blank , solvent and positive control group , re-spectively.Results Compared to solvent control group , Aescine in-creased the content of livermicrosome protein , however , it had no statisti-cal significance.Aescine had no effect on the content of CYP 450 , and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in solvent control group and Aescine group was comparable.Conclusions Aescine showed no effect on CYP 450 in rats.