CAJ | 학술논문

目的：研究二甲双胍微泡对人肺腺癌裸鼠移植瘤抑制的影响。方法构建肺腺癌裸鼠皮下移植瘤模型，表阿霉素溶液组和联合二甲双胍微泡化疗药物组，每2 d尾静脉注射5 mg· kg-1药物。正常组和空白脂质微泡组，每2 d尾静脉注射0.9％NaCl和空白脂质微泡（均0.1 mL/20 g）。给药后，测量肿瘤体积变化；在不同时间点（0.5，2，4，8，12，24 h）取大鼠组织。测定载药体系对裸鼠皮下肺腺癌移植瘤的肿瘤抑制率，观察HE染色检测移植瘤及裸鼠各脏器的形态学特征的变化。结果对大鼠肿瘤抑制率，联合二甲双胍微泡化疗药物组与表阿霉素溶液组分别为（86.79±3.13）％，（67.55±3.61）％。表阿霉素在心脏的聚积量，联合二甲双胍微泡疗药物组显著低于表阿霉素溶液组（ P＜0.05）。结论二甲双胍微泡能促进盐酸表阿霉素抑制人肺腺癌A549细胞体外增殖，具有相对靶向性。
목적：연구이갑쌍고미포대인폐선암라서이식류억제적영향。방법구건폐선암라서피하이식류모형，표아매소용액조화연합이갑쌍고미포화료약물조，매2 d미정맥주사5 mg· kg-1약물。정상조화공백지질미포조，매2 d미정맥주사0.9％NaCl화공백지질미포（균0.1 mL/20 g）。급약후，측량종류체적변화；재불동시간점（0.5，2，4，8，12，24 h）취대서조직。측정재약체계대라서피하폐선암이식류적종류억제솔，관찰HE염색검측이식류급라서각장기적형태학특정적변화。결과대대서종류억제솔，연합이갑쌍고미포화료약물조여표아매소용액조분별위（86.79±3.13）％，（67.55±3.61）％。표아매소재심장적취적량，연합이갑쌍고미포료약물조현저저우표아매소용액조（ P＜0.05）。결론이갑쌍고미포능촉진염산표아매소억제인폐선암A549세포체외증식，구유상대파향성。
Objective To investigate the inhibitory effect of metformin with lipid microbubbles on human lung adenocarcinoma in nude mice.Methods A model of lung adenocarcinoma xenograft in nude mice was established.Epirubicin group and epirubicin combined with metformin microbubble group ( ECMM) were intravenously injected into drugs at the dose of 5 mg · kg -1 every 2 d.Control group and blank microbubble group were intravenously injected into blank lipid microbubbles at the dose of 0.1 mL/20 g and 0.9%NaCl every 2 d.After treatment , tumor volume was detected.Tissues were obtained at the different time ( 0.5 , 2 , 4 , 8 , 12 , and 24 h ).The inhibition rate of drug carrier system on lung adenocarcinoma as well as morphological characteristics of HE stai-ning in the nude mice were detected.Results Tumor inhibition rates of epirubicin group and ECMM group were ( 67.55 ±3.61 )% and (86.79 ±3.13 )%, respectively.Accumulation of epirubicin in heart in ECMM group was significantly lower than that in epirubicin group ( P<0.05 ).Conclusion Combination with metformin microbubbles en-hances the inhibition of epirubicin on A 549 proliferation in vitro, indica-ting relative targeting.