CAJ | 학술논문

目的：研究乌苯美司对小鼠免疫细胞功能的影响。方法建立小鼠肝癌H22移植性肿瘤模型，观察其对小鼠肿瘤生长的影响；体外检测其对NK细胞的杀伤活性、巨噬细胞吞噬能力和NO分泌能力的影响；体内外刺激法测定其对脾淋巴细胞增殖作用；流式细胞法分析其对外周血肿瘤相关巨噬细胞（ TAMs ）和髓系衍生抑制细胞（ MDSCs ）以及脾脏T细胞、B细胞、NK细胞变化的影响。结果乌苯美司20 mg · kg · d-1连续给药7 d，对小鼠肝癌 H22抑瘤率为41.71％，对NK细胞的杀伤活性提高56.5％；能显著增强巨噬细胞的吞噬功能和NO的分泌能力；亦能促进淋巴细胞增殖，提高T淋巴细胞及其亚型CD8＋T细胞数量；使荷瘤鼠的TAMs和MDSCs恢复至正常水平。结论乌苯美司对小鼠免疫细胞功能具有增强作用。
목적：연구오분미사대소서면역세포공능적영향。방법건립소서간암H22이식성종류모형，관찰기대소서종류생장적영향；체외검측기대NK세포적살상활성、거서세포탄서능력화NO분비능력적영향；체내외자격법측정기대비림파세포증식작용；류식세포법분석기대외주혈종류상관거서세포（ TAMs ）화수계연생억제세포（ MDSCs ）이급비장T세포、B세포、NK세포변화적영향。결과오분미사20 mg · kg · d-1련속급약7 d，대소서간암 H22억류솔위41.71％，대NK세포적살상활성제고56.5％；능현저증강거서세포적탄서공능화NO적분비능력；역능촉진림파세포증식，제고T림파세포급기아형CD8＋T세포수량；사하류서적TAMs화MDSCs회복지정상수평。결론오분미사대소서면역세포공능구유증강작용。
Objective To study the effects of ubenimex on immunocyte functions of mice.Methods Antitumor activity of ubenimex was observed in implanted H22 mice.The killing activity of NK cells to target cell YAC-1, phagocytosis of peritoneal macrophage and the secretion of mac -rophage NO effect were carried out with CCK method , neutral red colori-metric method and Griess method , respectively.Lymphocyte proliferation test was done by stimulating methods in vitro and in vivo.Tumor associated macrophages(TAMs)and myeloid derived suppressor cells (MDSCs) in pe-ripheral blood and T lymphocytes , B lymphocytes and NK cell in spleen were determined by flow cytometry method.Results The 20 mg· kg-1 of ubenimex could depress the growth of H 22 and its tumor inhibitory rate was 41.71%.The killing activity of NK cells was increased by 56.5%.The 10μg· mL-1 of ubenimex significantly enhanced secretion of macrophage NO effect (P<0.01) and it also enhanced phagocytosis of peritoneal mac-rophage (P<0.05) at concentration of 100μg· mL-1.Besides the lym-phocyte proliferation was also strengthened (P<0.05).The number of T lymphocytes and CD 8+T cell in spleen was increased obviously ( P<0.01 or P<0.05).Ubenimex could adjust the number of TAMs and MDSCs in peripheral blood to normal level.Conclusion Ubenimex has antitumor growth activity through enhances non -specific immunity and specific immunity and suppresses the tumor associated cell.