CAJ | 학술논문

目的：观察雷公藤多苷（ TWP）对糖尿病肾病（ DN）大鼠肾脏结缔组织生长因子（ CTGF ）表达和排泄的影响。方法用腹腔注射链脲佐菌素的方法建立糖尿病大鼠模型。动物随机分为正常组、模型组、3个剂量（45，90，180 mg· kg-1） TWP组；用药8周末，检测大鼠血糖（ BG）、体重（ BW）、肾重（ KW）、肾重/体重（ KW/BW） B、24 h尿微量蛋白清除率（ UAER）、血尿素氮（ BUN）和肌酐清除率（ Ccr）；HE染色观察大鼠肾组织病理学变化；免疫组化检测肾皮质CT-GF蛋白表达。结果与模型组相比，TWP能明显改善DN大鼠一般情况，高剂量TWP组空腹BG明显降低（ P＜0.01），中、高剂量TWP组能使BW明显增加（P＜0.01），同时使 KW、KW/BW、24hUAER、BUN 及 Ccr 也明显降低（ P ＜0.01），使肾组织着色程度明显减轻，使CTGF表达显著下降（ P＜0.01），并使肾小球肥大、系膜细胞增生及肾小球变形病变均明显减轻。结论 TWP能明显改善肾脏功能，且与剂量相关，其机制可能与抑制肾脏 CTGF表达有关。
목적：관찰뢰공등다감（ TWP）대당뇨병신병（ DN）대서신장결체조직생장인자（ CTGF ）표체화배설적영향。방법용복강주사련뇨좌균소적방법건립당뇨병대서모형。동물수궤분위정상조、모형조、3개제량（45，90，180 mg· kg-1） TWP조；용약8주말，검측대서혈당（ BG）、체중（ BW）、신중（ KW）、신중/체중（ KW/BW） B、24 h뇨미량단백청제솔（ UAER）、혈뇨소담（ BUN）화기항청제솔（ Ccr）；HE염색관찰대서신조직병이학변화；면역조화검측신피질CT-GF단백표체。결과여모형조상비，TWP능명현개선DN대서일반정황，고제량TWP조공복BG명현강저（ P＜0.01），중、고제량TWP조능사BW명현증가（P＜0.01），동시사 KW、KW/BW、24hUAER、BUN 급 Ccr 야명현강저（ P ＜0.01），사신조직착색정도명현감경，사CTGF표체현저하강（ P＜0.01），병사신소구비대、계막세포증생급신소구변형병변균명현감경。결론 TWP능명현개선신장공능，차여제량상관，기궤제가능여억제신장 CTGF표체유관。
Objective To investigate the effect of Tripterygium wilfordii polyglycosidum ( TWP ) on the expression and excretion of connective tissue growth factor ( CTGF ).Methods The diabetes mellitus Sprague Dawley rats was induced by streptozotocin .The diabetic rats were random-ly divided in to five groups:normal group ,diabetic model group and three doses (45,90,180 mg · kg -1 ) of TWP groups.Blood glucose ( BG), body weight ( BW) , kidney weight ( KW) , KW/BW, 24 hours urinary al-bumin excretion rate ( UAER ) , blood urea nitrogen ( BUN ) , creatinine clearance ( Ccr) were measured at the end of the 8th week.The patholo-gy changing of the rat kidney was observed by HE staining , and the ex-pression of CTGF was determined by immunohistochemical assay.Re-sults Compared with model group , TWP can significantly improve the general situation of DN rats.TWP (180 mg· kg -1 ) can significantly de-creased fasting BG ( P<0.01 ).TWP ( 90 and 180 mg· kg -1 ) can sig-nificantly increase BW of rats ( P <0.01 ) , and TWP ( 90 and 180 mg · kg -1 ) also can significantly reduce KD , KW/BW, 24hUAER, BUN ( P<0.01 ).And the coloring of the renal tissue mitigate signifi-cantly, the expression of CTGF significantly decreased in TWP (90 and 180 mg· kg -1 ) groups; glomerular hypertrophy , mesangial cellproliferation and glomerular lesions all significantly reduce.Conclusion The TWP can obviously improve the renal function, and dose-related, and its mechanism may be correlated with inhibition on expression of CTGF in kindey.