CAJ | 학술논문

目的：研究山茱萸果核水提取物（ AEFCOC ）的降压作用及其作用机制。方法2 K2 C法建立大鼠肾性高血压模型；用大鼠离体胸主动脉环灌流，记录张力变化。结果 AEFCOC可剂量依赖性降低大鼠血压水平；0.03，0.1，0.3，1，2 mg· mL-1 AEFCOC可以剂量依赖性地舒张PE预收缩的内皮完整的大鼠胸主动脉环；NO合酶抑制剂L-NAME（1×10-4 mol· L-1）、环氧酶抑制剂Indo（1×10-5 mol· L-1）处理血管后，AEFCOC的舒张血管效应均被阻断（ P＜0.05）；去内皮时，AEFCOC（2 mg· mL-1）对PE预收缩的血管环始呈现舒张作用（P＜0.05）。结论 AEFCOC 可显著降低大鼠肾性高血压，可能与舒血管有关；其舒血管作用主要是内皮依赖性的，通过NO-鸟苷酸环化酶途径和环氧酶途径。
목적：연구산수유과핵수제취물（ AEFCOC ）적강압작용급기작용궤제。방법2 K2 C법건립대서신성고혈압모형；용대서리체흉주동맥배관류，기록장력변화。결과 AEFCOC가제량의뢰성강저대서혈압수평；0.03，0.1，0.3，1，2 mg· mL-1 AEFCOC가이제량의뢰성지서장PE예수축적내피완정적대서흉주동맥배；NO합매억제제L-NAME（1×10-4 mol· L-1）、배양매억제제Indo（1×10-5 mol· L-1）처리혈관후，AEFCOC적서장혈관효응균피조단（ P＜0.05）；거내피시，AEFCOC（2 mg· mL-1）대PE예수축적혈관배시정현서장작용（P＜0.05）。결론 AEFCOC 가현저강저대서신성고혈압，가능여서혈관유관；기서혈관작용주요시내피의뢰성적，통과NO-조감산배화매도경화배양매도경。
Objective To investigate the lowering blood pressure effect of aquatic extraction of Fructus Corni Officinalis Core ( AEFCOC ) , and to study the underlying mechanisms.Methods Renal hypertension was induced by two kidney two clip method (2K2C), and blood pressure was dynamic recording.Isolated rat thoracic aorta rings were performed in or-gan bath , and the tention was record.Results Compared with sham -operated group , the blood pressure was markedly elevated in model groups , while AEFCOC treatment for 4 weeks could relieve hypertention.AEFCOC (0.03,0.1,0.3,1,2 mg· mL-1 ) concentration dependently caused endothelium -dependent relaxation in vessels precontracted with phenylephrine (PE), which was abolished by either 1 ×10 -4 mol· L-1 NG-nitro -L -arginine methyl ester ( L -NAME ) or 1 ×10 -5 mol· L-1 COX inhibitor indomethacin ( Indo) ( P<0.05 ).On de-en-dothelialized vessels precontracted with PE , AEFCOC 2 mg · mL-1 caused it relaxation.Conclusion AEFCOC could significantly decrease the blood pressure in dose -dependent manner , which is possibly associ-ated with vasodilator action.AEFCOC could relax the rat thoracic aorta rings mainly with endothelium -dependent manner , involving the NO -GC pathway and COX pathway in vascular endothelium cells.