中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2014年
7期
588-590
,共3页
杨小霞%李国前%王杰华%洪诸权%庄一虹%潘莹
楊小霞%李國前%王傑華%洪諸權%莊一虹%潘瑩
양소하%리국전%왕걸화%홍제권%장일홍%반형
丁苯酞%脑缺血再灌注%逆转录聚合酶链反应法
丁苯酞%腦缺血再灌註%逆轉錄聚閤酶鏈反應法
정분태%뇌결혈재관주%역전록취합매련반응법
dl -3 -n -butylphthalide%cerebral ischemia -reperfu-sion%reverse transcription -polymerase chain reaction
目的：观察丁苯酞（ NBP）对局灶性脑缺血再灌注损伤大鼠脑组织Fas和FasL表达的影响。方法30只SD大鼠随机分为假手术组（ Sham组）、脑缺血再灌注组（ I/R组）和丁苯酞治疗组（ NBP组）。线栓法制作大鼠大脑中动脉脑缺血再灌注模型，于缺血2 h再灌注24 h后断头取脑，免疫组织化学法检测缺血区脑组织Fas和FasL的蛋白表达，逆转录聚合酶链反应（ RT-PCR）法检测缺血区脑组织Fas和FasL的mRNA表达。结果与Sham组相比， I/R组缺血区脑组织Fas和FasL的蛋白和mRNA的表达均显著增多；与I/R组相比，NBP组缺血区脑组织Fas和FasL的蛋白和mRNA的表达均显著下调，差异均有统计学意义（ P＜0.05）。结论丁苯酞对大鼠脑缺血再灌注损伤的保护作用，其机制可能与抑制Fas和FasL的表达有关。
目的：觀察丁苯酞（ NBP）對跼竈性腦缺血再灌註損傷大鼠腦組織Fas和FasL錶達的影響。方法30隻SD大鼠隨機分為假手術組（ Sham組）、腦缺血再灌註組（ I/R組）和丁苯酞治療組（ NBP組）。線栓法製作大鼠大腦中動脈腦缺血再灌註模型，于缺血2 h再灌註24 h後斷頭取腦，免疫組織化學法檢測缺血區腦組織Fas和FasL的蛋白錶達，逆轉錄聚閤酶鏈反應（ RT-PCR）法檢測缺血區腦組織Fas和FasL的mRNA錶達。結果與Sham組相比， I/R組缺血區腦組織Fas和FasL的蛋白和mRNA的錶達均顯著增多；與I/R組相比，NBP組缺血區腦組織Fas和FasL的蛋白和mRNA的錶達均顯著下調，差異均有統計學意義（ P＜0.05）。結論丁苯酞對大鼠腦缺血再灌註損傷的保護作用，其機製可能與抑製Fas和FasL的錶達有關。
목적：관찰정분태（ NBP）대국조성뇌결혈재관주손상대서뇌조직Fas화FasL표체적영향。방법30지SD대서수궤분위가수술조（ Sham조）、뇌결혈재관주조（ I/R조）화정분태치료조（ NBP조）。선전법제작대서대뇌중동맥뇌결혈재관주모형，우결혈2 h재관주24 h후단두취뇌，면역조직화학법검측결혈구뇌조직Fas화FasL적단백표체，역전록취합매련반응（ RT-PCR）법검측결혈구뇌조직Fas화FasL적mRNA표체。결과여Sham조상비， I/R조결혈구뇌조직Fas화FasL적단백화mRNA적표체균현저증다；여I/R조상비，NBP조결혈구뇌조직Fas화FasL적단백화mRNA적표체균현저하조，차이균유통계학의의（ P＜0.05）。결론정분태대대서뇌결혈재관주손상적보호작용，기궤제가능여억제Fas화FasL적표체유관。
Objective To investigate the effects of dl -3 -n -bu-tylphthalide on the expression of Fas and FasL in rats with focal cerebral ischemia -reperfusion.Methods Thirty rats were randomly assigned into three groups:Sham operation group (Sham group),focal cerebral is-chemia-reperfusion group ( I/R group ) and dl-3-n-butylphthalide group ( NBP group ).The middle cerebral artery occlusion reperfusion model was made by the suture method (ischemia for 2 hours, and reper-fusion for 24 hours ).The level of Fas and FasL expression were meas-ured by immunohistochemistry and reverse transcription -polymerase chain reaction(RT-PCR).Results Compared with sham group, the expressions of Fas and FasL as well as the mRNA were significantly up -regulated in I/R group ( P<0.05 ).Compared with I/R group, the ex-pressions of Fas and FasL as well as the mRNA were significantly down -regulated in the NBP group ( P<0.05 ).Conclusion The protection effect of dl-3-n-butylphthalide on rats with focal cerebral ischemia -reperfusion may be related to the inhibition of the expression of Fas and FasL.
