CAJ | 학술논문

目的：考察CYP2C19*2（G681A）和CYP2C19*3（G636A）基因多态性与急性淋巴细胞白血病（ ALL）易感性及甲氨蝶呤（ MTX）血清浓度的相关性。方法收集283名健康对照受试者和91例ALL患儿的外周血，提取DNA。用聚合酶链反应-限制性片断长度多态性法检测CYP2C19*2和CYP2C19*3基因型，用荧光偏振免疫分析法测定24，42 h MTX血清浓度。结果 ALL患儿与健康对照人群的CYP2C19*2和CYP2C19*3基因型与等位基因分布频率相近，与野生等位基因相比，变异等位基因（ A ）降低了ALL的发病风险，但差异无统计学意义。 CYP2C19*2和CYP2C19*3各基因型组的ALL患儿于24，42 h MTX浓度与剂量比值（ C/D ratio）比较，差异无统计学意义。结论 CYP2C19*2和CYP2C19*3基因多态性与ALL的发病风险和MTX血清浓度无明显相关性。
목적：고찰CYP2C19*2（G681A）화CYP2C19*3（G636A）기인다태성여급성림파세포백혈병（ ALL）역감성급갑안접령（ MTX）혈청농도적상관성。방법수집283명건강대조수시자화91례ALL환인적외주혈，제취DNA。용취합매련반응-한제성편단장도다태성법검측CYP2C19*2화CYP2C19*3기인형，용형광편진면역분석법측정24，42 h MTX혈청농도。결과 ALL환인여건강대조인군적CYP2C19*2화CYP2C19*3기인형여등위기인분포빈솔상근，여야생등위기인상비，변이등위기인（ A ）강저료ALL적발병풍험，단차이무통계학의의。 CYP2C19*2화CYP2C19*3각기인형조적ALL환인우24，42 h MTX농도여제량비치（ C/D ratio）비교，차이무통계학의의。결론 CYP2C19*2화CYP2C19*3기인다태성여ALL적발병풍험화MTX혈청농도무명현상관성。
Objective To investigate the associations between CYP2C19*2 (G681A) and CYP2C19*3 (G636A) polymorphisms with the risk to develop acute lymphoblastic leukemia ( ALL ) and serum concentra-tions of methotrexate ( MTX).Methods Peripheral blood samples were obtained from healthy subjects as control samples ( n=283 ) and children with acute lymphoblastic leukemia ( n =91 ) to extract genome DNA.Polymerase chain reaction-restriction fragment length polymorphism was used to detect the genotypes of CYP2C19*2 and CYP2C19*3 polymor-phisms.Fluorescence polarization immunoassay was employed to deter-mine the serum concentrations of MTX at the time of 24 h and 42 h.Results There were similar frequencies of genotypes and alleles at CYP2 C19*2 and CYP2 C19*3 in healthy subjects and ALL children.Compared with wid alleles , variant alleles ( A) lowered the risk of ALL , but there was no statistical difference.There were no significant differ-ences in the dose-adjusted serum concentration ( C/D ratio) of MTX at the 24 h and 42 h among respective genotype groups at CYP2 C19*2 and CYP2C19*3.Conclusion CYP2C19*2 and CYP2C19*3 polymorphisms are not associated with the risk to develop ALL and serum concentrations of MTX.