CAJ | 학술논문

应用重组人甲状腺激素受体(hTR)基因酵母快速测试方法，检测北京官厅水库水体类/抗甲状腺激素干扰效应，并分析其地理分布、季节分布特征以及水体甲状腺激素干扰化合物(TDCs)组成特征.结果表明：官厅水库水体检出了显著的抗甲状腺激素干扰效应，与其它研究区域相比，效应处于较低到中等水平；入库河流对库区水质产生显著影响，且入库河流水质受季节影响显著，因此，库区水体不同季节甲状腺激素干扰水平存在差异；C18固相萃取柱对抗甲状腺激素干扰效应的去除率在61.9%~92.3%，表明官厅水库水体中的甲状腺激素抑制活性物质多集中在有机组分，加入30mg/L的EDTA后TR抑制活性显著降低，表明官厅水库水体中TDCs除有机化合物外可能还包含重金属类无机化合物.
응용중조인갑상선격소수체(hTR)기인효모쾌속측시방법，검측북경관청수고수체류/항갑상선격소간우효응，병분석기지리분포、계절분포특정이급수체갑상선격소간우화합물(TDCs)조성특정.결과표명：관청수고수체검출료현저적항갑상선격소간우효응，여기타연구구역상비，효응처우교저도중등수평；입고하류대고구수질산생현저영향，차입고하류수질수계절영향현저，인차，고구수체불동계절갑상선격소간우수평존재차이；C18고상췌취주대항갑상선격소간우효응적거제솔재61.9%~92.3%，표명관청수고수체중적갑상선격소억제활성물질다집중재유궤조분，가입30mg/L적EDTA후TR억제활성현저강저，표명관청수고수체중TDCs제유궤화합물외가능환포함중금속류무궤화합물.
A rapid recombinant human thyroid hormone receptor (hTR) gene yeast bioassay was used to evaluate the thyroid disrupting effects in the water from the Guanting Reservoir (GR), Beijing, China. Furthermore, the geographic and seasonal distribution, and thyroid disrupting chemicals (TDCs) composition characteristics of water were analysed. The results revealed that water samples had TR antagonistic activities. The Guanting Reservoir water displayed a low to moderate effect on thyroid hormone signalling, which was impacted by the input river. The effects of the input river were significantly affected by season, so the effects of reservoir water were temporally variable. A solid phase extraction by C18cartridges was used to separate the organic extracts, which eliminated 61.9%~92.3% antagonistic activities, suggesting the organic extracts may play a major role in the TR disruption effect of the water. After addition of EDTA (30mg/L) to the residual water samples, the antagonistic activities decreased significantly, showing the metals might contribute to the TR antagonistic activity.