CAJ | 학술논문

目的：建立人膀胱癌 T24细胞裸鼠移植瘤模型，探讨外源性表达 VEGF165b 对其生长的影响及其机制。方法分别移植未转染和转染 VEGF165b 表达载体的人膀胱癌 T24细胞到裸鼠膀胱内，28 d 使处死动物，称量肿瘤重量，测定肿瘤体积。Western blot 法检测肿瘤 VEGF165b、p-VEGFR2、AKT、p-AKT 蛋白表达。结果转染 pcD-NA-VEGF165b 组肿瘤重量和体积与未转染组和转染 pcDNA3．0组相比明显降低（P ＜0．05），而未转染组和转染pcDNA3．0组比较无统计学差异（P ＞0．05）。Western blot 结果显示，转染 pcDNA-VEGF165b 组与未转染组和转染pcDNA3．0组比较，VEGF165b 表达增高，p-VEGFR2、p-AKT 表达降低，而未转染组和转染 pcDNA3．0组间无明显差异。结论外源性表达 VEGF165b 可通过抑制 VEGFR2信号通路传导而明显抑制人膀胱癌裸鼠移植瘤生长。
목적：건립인방광암 T24세포라서이식류모형，탐토외원성표체 VEGF165b 대기생장적영향급기궤제。방법분별이식미전염화전염 VEGF165b 표체재체적인방광암 T24세포도라서방광내，28 d 사처사동물，칭량종류중량，측정종류체적。Western blot 법검측종류 VEGF165b、p-VEGFR2、AKT、p-AKT 단백표체。결과전염 pcD-NA-VEGF165b 조종류중량화체적여미전염조화전염 pcDNA3．0조상비명현강저（P ＜0．05），이미전염조화전염pcDNA3．0조비교무통계학차이（P ＞0．05）。Western blot 결과현시，전염 pcDNA-VEGF165b 조여미전염조화전염pcDNA3．0조비교，VEGF165b 표체증고，p-VEGFR2、p-AKT 표체강저，이미전염조화전염 pcDNA3．0조간무명현차이。결론외원성표체 VEGF165b 가통과억제 VEGFR2신호통로전도이명현억제인방광암라서이식류생장。
Objective Establish the transplantation tumor model of human bladder cancer T24 cells in nude mice and explore the exogenous expression of VEGF165b on its growth and its mechanism.Methods Human bladder cancer T24 cells were transplant into bladders of nude mice.The mice were killed in 28d after transplantation.Western blot was used to confirm the protein expression of VEGF165b、p-VEGFR2、AKT、p-AKT.Results Compared with untreat-ed group and pcDNA3.0 group,the weight and volume of tumor tissue were decreased in pcDNA-VEGF165b group(P<0.05).But there was no significant difference between untreated group and pcDNA3.0 group(P >0.05).The pro-tein expressions indicate that compared with untreated group and pcDNA3.0 group,VEGF165b was increased;p-VEG-FR2 and p-AKT were decreased.But there was no significant difference between untreated group and pcDNA3.0 group.Conclusion Exogenous expression of VEGF165b suppresses signaling pathway of VEGFR2 and inhibits the growth of nude mice model of bladder cancer.