CAJ | 학술논문

目的：研究小肠结肠炎耶尔森菌（Y ．e）人畜感染情况，探索传染来源。方法采集腹泻病人粪便标本和猪咽拭子进行Y ．e常规分离鉴定。对分离株进行血清学分型，用PCR方法检测毒力基因，用脉冲场凝胶电泳（PFGE ）进行分子分型。结果从150例腹泻病人中分离到Y ．e 3株，其中1株血清型为O∶3；从该O∶3株病患老家采集的222份猪咽拭子分离到Y ．e 14株，都为O∶3血清型；PFGE分子分型显示病人体内分离到的致病Y ．e与家猪分离株有较高的同源性。结论证实1例小肠结肠炎耶尔森菌从家猪传染人的病例，并发现Y．e在治愈病人体内至少存在了2个月。
목적：연구소장결장염야이삼균（Y ．e）인축감염정황，탐색전염래원。방법채집복사병인분편표본화저인식자진행Y ．e상규분리감정。대분리주진행혈청학분형，용PCR방법검측독력기인，용맥충장응효전영（PFGE ）진행분자분형。결과종150례복사병인중분리도Y ．e 3주，기중1주혈청형위O∶3；종해O∶3주병환로가채집적222빈저인식자분리도Y ．e 14주，도위O∶3혈청형；PFGE분자분형현시병인체내분리도적치병Y ．e여가저분리주유교고적동원성。결론증실1례소장결장염야이삼균종가저전염인적병례，병발현Y．e재치유병인체내지소존재료2개월。
Yersinia enterocolitica infection in humans and animals was investigated in this study to explore the source of infection .We collected samples from patients and pigs ,and then the strains were conducted to undergo serotyping ,virulence gene detection and PFGE molecular typing .Of the 150 patients samples ,3 were tested positive for Yersinia enterocolitica ,and one of them was O∶3 .Of the 222 pigs samples ,14 were tested positive for Yersinia enterocolitica ,and all of them were O∶3 .PFGE molecular typing showed that the Yersinia enterocolitica from patient and pig had high homology .Our study con-firmed that Yersinia enterocolitica from pigs could infect people and find that it could exist in the cured patient for at least two months .