CAJ | 학술논문

目的：了解河南省信阳市动物中新布尼亚病毒感染情况，为研究其传播途径和防治策略奠定基础。方法在信阳市商城县和光山县采集动物血液，应用荧光RT-PCR方法检测血液标本中是否携带新布尼亚病毒核酸，采用双抗原夹心ELISA方法检测新布尼亚病毒总抗体。结果在调查点采集到5种动物血清标本合计312份，结果均未检测到新布尼亚病毒核酸；141份动物血清检测到新布尼亚病毒总抗体，总体阳性率为45．19％，鼠、牛、羊、猪和狗分别为1．06％，100．00％、76．27％、3．57％和75．00％，5种动物的抗体阳性率有统计学意义。结论河南省信阳市牛、羊、狗等家养动物中存在新布尼亚病毒抗体，可能为该病毒的储存宿主。
목적：료해하남성신양시동물중신포니아병독감염정황，위연구기전파도경화방치책략전정기출。방법재신양시상성현화광산현채집동물혈액，응용형광RT-PCR방법검측혈액표본중시부휴대신포니아병독핵산，채용쌍항원협심ELISA방법검측신포니아병독총항체。결과재조사점채집도5충동물혈청표본합계312빈，결과균미검측도신포니아병독핵산；141빈동물혈청검측도신포니아병독총항체，총체양성솔위45．19％，서、우、양、저화구분별위1．06％，100．00％、76．27％、3．57％화75．00％，5충동물적항체양성솔유통계학의의。결론하남성신양시우、양、구등가양동물중존재신포니아병독항체，가능위해병독적저존숙주。
To investigate the animals infection situation of novel bunyavirus in Xinyang City ,Henan Province ,China , animal serum samples such as cattle ,dog ,swine ,mice were collected in Shangcheng County and Guangshan County in Xinyang City .All the serum samples were detected by novel bunyavirus ELISA and real time RT-PCR method .A total of 292 animal serum samples were collected including 5 kinds of animals .The result of all the animal serum samples were negative by using real time RT-PCR ,and the positive rate was 45 .19% (141/312) by ELISA method .Of the 5 animal serum samples including mice ,cattle ,goats ,swine and dogs ,the positive rate were detected to be 1 .06% ,100 .00% ,76 .27% ,3 .57% ,and 75 .00%respectively .There was significant difference in results among 5 kind of animal serum antibodies .Animals such as cattle and dog may be the host of novel bunyavirus which were detected novel bunyavirus antibodies in cattle and dog in Xinyang City , Henan Province ,China .