CAJ | 학술논문

目的：对脉冲场凝胶电泳方法（PFGE）和多位点可变数目串联重复序列分型（MLVA）两种分子分型方法在布鲁氏菌分型研究中的应用进行比较和评价。方法采用PFGE和MLVA分子分型方法对60株布鲁氏菌地方株和3株标准菌株（16M、544A、1330S）进行分型比较。结果63株菌株间PFGE相似性系数介于72．1％～100％，在94．4％相似水平可区分羊、猪、牛3个种，在100．0％相似水平上可分为14个群29种PFGE型别，分辨力为0．9575；MLVA相似性系数介于16．9％～100％，在52．3％相似水平可区分羊、猪、牛3个种，在100．0％相似水平上可分为8个群47种MLVA型别，分辨力为0．9852。结论PFGE和MLVA均可在一定的相似系数从种的水平区分羊、猪、牛3个种，但不能区分同种异型；两种方法均可用于布鲁氏菌的分子分型，MLVA较PFGE具有稍高的分辨能力。
목적：대맥충장응효전영방법（PFGE）화다위점가변수목천련중복서렬분형（MLVA）량충분자분형방법재포로씨균분형연구중적응용진행비교화평개。방법채용PFGE화MLVA분자분형방법대60주포로씨균지방주화3주표준균주（16M、544A、1330S）진행분형비교。결과63주균주간PFGE상사성계수개우72．1％～100％，재94．4％상사수평가구분양、저、우3개충，재100．0％상사수평상가분위14개군29충PFGE형별，분변력위0．9575；MLVA상사성계수개우16．9％～100％，재52．3％상사수평가구분양、저、우3개충，재100．0％상사수평상가분위8개군47충MLVA형별，분변력위0．9852。결론PFGE화MLVA균가재일정적상사계수종충적수평구분양、저、우3개충，단불능구분동충이형；량충방법균가용우포로씨균적분자분형，MLVA교PFGE구유초고적분변능력。
To compare and evaluate the discriminatory ability and potential value of pulsed field gel electrophoresis (PF-GE) and multiple locus VNTRs analysis (MLVA) on the genotyping of Brucella ,a total of 60 strains of Brucella and three standards (16M ,544A ,1330S) were genotyped simultaneously by PFGE and MLVA .The result indicated that the similarity coefficient among the 63 isolates was from 72 .1-100 .0% by PFGE ,and could distinguish three species of B .melitensis ,B .su-is and B .abortus at the similarity level of 94 .4% .There were 14 clusters and 29 PFGE types identified by PFGE with discrim-inatory index (DI) of 0 .957 5 at the similarity level of 100% ;the similarity coefficient among the 63 isolates was from 16 .9-100 .0% by MLVA ,and could distinguish three species of Brucella at the similarity level of 52 .3% .There were 8 clusters and 47 MLVA types identified by MLVA with discriminatory index (DI) of 0 .985 2 at the similarity level of 100% .It's suggested that PFGE and MLVA could be used to distinguish three species of Brucella in the similarity coefficient of certain ,but could not effectively distinguish the type in the same species .Both of these two methods could be used for Brucella molecular typing , but MLVA is better than PFGE for its relatively higher discriminating ability .