CAJ | 학술논문

目的：观察姜黄素对毒性休克综合征毒素-1（TSST-1）诱导的小鼠脾细胞炎症因子的影响，为进一步探讨姜黄素对炎症性休克的作用提供依据。方法采用乳酸脱氢酶（LDH ）释放法检测不同剂量的 TSST-1和姜黄素的细胞毒作用， ELISA和流式细胞术检测相关炎症因子。结果所选剂量 TSST-1和姜黄素均未发现有明显细胞毒作用。TSST-1诱导脾细胞产生了大量的IFN-γ和IL-2，产生了少量的TNF-α，未能诱导出IL-1β、IL-6和IL-12。TSST-1诱导的Th1细胞因子产生不完全依赖于IL-12。姜黄素对IFN-γ和TNF-α的抑制作用与剂量有关，在15μmol/L抑制效果最显著（P＜0．05），但对IL-2无显著抑制作用（P＞0．05）。结论姜黄素能显著降低TSST-1诱导小鼠脾细胞产生的IFN-γ和TNF-α，但对 T细胞增殖无明显抑制作用。
목적：관찰강황소대독성휴극종합정독소-1（TSST-1）유도적소서비세포염증인자적영향，위진일보탐토강황소대염증성휴극적작용제공의거。방법채용유산탈경매（LDH ）석방법검측불동제량적 TSST-1화강황소적세포독작용， ELISA화류식세포술검측상관염증인자。결과소선제량 TSST-1화강황소균미발현유명현세포독작용。TSST-1유도비세포산생료대량적IFN-γ화IL-2，산생료소량적TNF-α，미능유도출IL-1β、IL-6화IL-12。TSST-1유도적Th1세포인자산생불완전의뢰우IL-12。강황소대IFN-γ화TNF-α적억제작용여제량유관，재15μmol/L억제효과최현저（P＜0．05），단대IL-2무현저억제작용（P＞0．05）。결론강황소능현저강저TSST-1유도소서비세포산생적IFN-γ화TNF-α，단대 T세포증식무명현억제작용。
In the present study ,we aimed to observe the effect of curcumin on TSST-1-induced inflammatory cytokines in splenocytes of mouse and provide evidence for the further study on the effect of curcumin on inflammatory shock .Lactate de-hydrogenase (LDH) release assay was used to determine cytotoxicity of different doses of TSST-1 and curcumin .Inflammatory cytokines were determined by ELISA and flow cytometry .The doses of TSST-1 and curcumin we used in the present study did not cause significant cytotoxicity .TSST-1 induced higher level of IFN-γand IL-2 production but relatively lower level of TNF-α.The production of IL-1β,IL-6 and IL-12 was undetectable .TSST-1 induced Th1 cytokines (IFN-γand IL-2) were not IL-12-dependent which was different from LPS-induced IFN-γ.Curcumin significantly reduced IFN-γand TNF-αproduction at the concentration of 15 umol/L (P<0 .05) ,but had no effect on IL-2 production (P>0 .05) .It’s suggested that curcumin could significantly inhibit the production of IFN-γand TNF-αby splenocytes induced by TSST-1 ,but could not affect the prolifera-tion of T cells .