CAJ | 학술논문

目的：监测分析灰仓鼠真菌多样性。方法60只成年灰仓鼠来自中国新疆地区，安乐死后剖解采集标本。应用TaqMan MGB探针实时荧光定量PCR、核糖体克隆测序、真菌培养鉴定技术对灰仓鼠真菌多样性进行分析。结果鉴定出60株真菌，包括白假丝酵母、阿萨丝孢酵母、烟曲霉、黑曲霉、聚多曲霉、日本曲霉、焦曲霉、杂色曲霉、产黄青霉、宛氏拟青霉、黄灰青霉、好食脉孢菌、间型脉孢菌、芽枝状枝孢。灰仓鼠携带的真菌多为人兽共患病病原体。结果显示，曲霉菌是最主要优势菌群，青霉菌是次优势菌群。这些菌株对制霉菌素、克霉唑、沃尔康唑等药物敏感。结论本研究首次应用 T aqM an M GB探针实时荧光定量PCR和核糖体克隆测序、真菌鉴定培养技术能有效分析灰仓鼠真菌多样性。研究结果为我国灰仓鼠微生物学监测及质量标准制定提供了科学依据。本研究第一个广泛报道了灰仓鼠真菌菌群多样性。
목적：감측분석회창서진균다양성。방법60지성년회창서래자중국신강지구，안악사후부해채집표본。응용TaqMan MGB탐침실시형광정량PCR、핵당체극륭측서、진균배양감정기술대회창서진균다양성진행분석。결과감정출60주진균，포괄백가사효모、아살사포효모、연곡매、흑곡매、취다곡매、일본곡매、초곡매、잡색곡매、산황청매、완씨의청매、황회청매、호식맥포균、간형맥포균、아지상지포。회창서휴대적진균다위인수공환병병원체。결과현시，곡매균시최주요우세균군，청매균시차우세균군。저사균주대제매균소、극매서、옥이강서등약물민감。결론본연구수차응용 T aqM an M GB탐침실시형광정량PCR화핵당체극륭측서、진균감정배양기술능유효분석회창서진균다양성。연구결과위아국회창서미생물학감측급질량표준제정제공료과학의거。본연구제일개엄범보도료회창서진균균군다양성。
To monitor and analyze Cricetulus migratorius fungal diversity ,60 adult Cricetulus migratorius brought from Xinjiang region of China were dissected after being euthanized and the specimens were collected .Fungal diversity research was carried out by TaqMan MGB probe real-time fluorescence quantitative PCR ,the ribosome cloning sequencing ,and fungal cul-ture identification techniques .The 60 fungal isolates were characterized from Cricetulus migratorius ,including Candida albi-cans ,Trichosporonasahii ,Aspergillus fumigatus ,Aspergillusniger ,Aspergillussydowii ,Aspergillus japonicus ,Asper-gillus ustus ,Aspergillus versicolor ,Penicillium chrysogenum ,Paecilomyces variotii ,Penicillium aurantiogriseum ,Neuros-pora sitophila ,Neurospora intermedia ,and Cladosporium cladosporioides .Many of them associated with grey hamster as zoonotic pathogens .The results showed that the most dominant fungal group was Aspergillus ,and Penicillium followed by it . These fungi were susceptible to nystatin ,clotrimazole and voriconazole .It’s indicated that application of TaqMan MGB probe real-time fluorescence quantitative PCR ,the ribosome cloning sequencing ,and fungal culture identification techniques could ef-fectively analyze Cricetulus migratorius .The results could provide a scientific basis for Cricetulus migratorius microbiological monitoring and quality standard establishment in China .Overall ,the findings of the present study constitute ,to the authors’ knowledge ,the first extensive report on the diversity of fungal flora associated with Cricetulus migratorius .