中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2014年
7期
688-691
,共4页
王琳%翁丽珍%陈晓红%黄明翔%李学玲%林剑东%郭志平%熊丽君%刘坦业
王琳%翁麗珍%陳曉紅%黃明翔%李學玲%林劍東%郭誌平%熊麗君%劉坦業
왕림%옹려진%진효홍%황명상%리학령%림검동%곽지평%웅려군%류탄업
肺结核%肺部炎症%鉴别诊断%蛋白指纹图谱技术%实验室诊断技术%免疫组质谱
肺結覈%肺部炎癥%鑒彆診斷%蛋白指紋圖譜技術%實驗室診斷技術%免疫組質譜
폐결핵%폐부염증%감별진단%단백지문도보기술%실험실진단기술%면역조질보
pulmonary tuberculosis%pneumonia%differential diagnosis%protein fingerprinting technology%laboratory diagnostic methods%immunomic mass spectrometry
目的:探索应用蛋白质指纹图谱技术于菌阴肺结核与肺炎的鉴别诊断。方法从本院临床病例中,选择菌阴肺结核和肺炎患者及健康者各60例,应用表面加强激光解吸电离飞行时间质谱技术(SELDI/ToF-Ms)和蛋白芯片技术检测血清蛋白,并应用Ciphergen蛋白芯片3.1.1软件进行比较,分析其相关蛋白峰值并进行统计学处理。结果对180例菌阴肺结核、肺炎患者、健康者的血清蛋白指纹图谱数据进行比较,发现有5个蛋白峰(1028.49、4796.56、7564.77、8048.02、11526.75 m/z )存在显著的差异,有统计学意义( P<0.01)。由这5个蛋白峰组成的诊断模型鉴别诊断菌阴肺结核与肺炎的总有效率84.2%(101/120),敏感性与特异性分别为82.5%(52/63),85.9%(49/57);阳性预测值86.7%(52/60),阴性预测值为81.7%(49/60)。诊断模型在判别肺炎、菌阴肺结核患者与健康者之间,总有效率达89.4%(161/180),特异性为100%(60/60),灵敏度为84.2%(101/120),阳性预测值100%(101/101),阴性预测值75.9%(60/79)。结论蛋白质指纹图谱技术具有方法简便、检测快速,标本用量少的优点,是筛选结核病特异性标志物的有效手段,通过蛋白质指纹图谱技术检测,发现了具有良好鉴别诊断的“诊断模型”。
目的:探索應用蛋白質指紋圖譜技術于菌陰肺結覈與肺炎的鑒彆診斷。方法從本院臨床病例中,選擇菌陰肺結覈和肺炎患者及健康者各60例,應用錶麵加彊激光解吸電離飛行時間質譜技術(SELDI/ToF-Ms)和蛋白芯片技術檢測血清蛋白,併應用Ciphergen蛋白芯片3.1.1軟件進行比較,分析其相關蛋白峰值併進行統計學處理。結果對180例菌陰肺結覈、肺炎患者、健康者的血清蛋白指紋圖譜數據進行比較,髮現有5箇蛋白峰(1028.49、4796.56、7564.77、8048.02、11526.75 m/z )存在顯著的差異,有統計學意義( P<0.01)。由這5箇蛋白峰組成的診斷模型鑒彆診斷菌陰肺結覈與肺炎的總有效率84.2%(101/120),敏感性與特異性分彆為82.5%(52/63),85.9%(49/57);暘性預測值86.7%(52/60),陰性預測值為81.7%(49/60)。診斷模型在判彆肺炎、菌陰肺結覈患者與健康者之間,總有效率達89.4%(161/180),特異性為100%(60/60),靈敏度為84.2%(101/120),暘性預測值100%(101/101),陰性預測值75.9%(60/79)。結論蛋白質指紋圖譜技術具有方法簡便、檢測快速,標本用量少的優點,是篩選結覈病特異性標誌物的有效手段,通過蛋白質指紋圖譜技術檢測,髮現瞭具有良好鑒彆診斷的“診斷模型”。
목적:탐색응용단백질지문도보기술우균음폐결핵여폐염적감별진단。방법종본원림상병례중,선택균음폐결핵화폐염환자급건강자각60례,응용표면가강격광해흡전리비행시간질보기술(SELDI/ToF-Ms)화단백심편기술검측혈청단백,병응용Ciphergen단백심편3.1.1연건진행비교,분석기상관단백봉치병진행통계학처리。결과대180례균음폐결핵、폐염환자、건강자적혈청단백지문도보수거진행비교,발현유5개단백봉(1028.49、4796.56、7564.77、8048.02、11526.75 m/z )존재현저적차이,유통계학의의( P<0.01)。유저5개단백봉조성적진단모형감별진단균음폐결핵여폐염적총유효솔84.2%(101/120),민감성여특이성분별위82.5%(52/63),85.9%(49/57);양성예측치86.7%(52/60),음성예측치위81.7%(49/60)。진단모형재판별폐염、균음폐결핵환자여건강자지간,총유효솔체89.4%(161/180),특이성위100%(60/60),령민도위84.2%(101/120),양성예측치100%(101/101),음성예측치75.9%(60/79)。결론단백질지문도보기술구유방법간편、검측쾌속,표본용량소적우점,시사선결핵병특이성표지물적유효수단,통과단백질지문도보기술검측,발현료구유량호감별진단적“진단모형”。
To explore the application of protein fingerprint technique and differential diagnosis in bacteriological negative pulmonary tuberculosis and pneumonia ,60 patients with bacteriological negative pulmonary tuberculosis ,60 patients with pneumonia ,and 60 healthy volunteers were selected from known clinical cases .Surface strengthening laser desorption ioniza-tion time of flight mass spectrometry (SELDI ToF Ms) and protein chip technology were applied to detect serum proteins ,and analyze their protein peaks by Ciphergen protein chip 3 .1 .1 software .Comparison of the serum protein fingerprinting data from the pool of 180 patients and healthy volunteers showed significant difference in 5 protein peaks (1 028 .49 ,4 796 .56 ,7 564 .77 , 8 048 .02 ,and 11 526 .75 m/z) identified between pulmonary tuberculosis and pneumonia (P<0 .01) .The total effective rate of the 5 protein peaks as a diagnosis model for differential diagnosis of bacteriological negative pulmonary tuberculosis and pneumonia was 84 .2% (101/120) ,the specificity was 82 .5% (52/63) ,the sensitivity was 85 .9% (49/57) ,the positive pre-dictive value was 86 .7% (52/60) ,and the negative predictive value was 81 .7% (49/60) .The total effective rate of the diagno-sis model for differential diagnosis of bacteriological negative pulmonary tuberculosis ,pneumonia and healthy volunteers was 89 .4% (161/180) .The specificity was 100% (60/60) ,the sensitivity was 84 .2% (101/120) ,the positive predictive value was 100% (101/101) ,and the negative predictive value was 75 .9% (60/79) .Protein fingerprinting technology is advanta-geous of being a simple method ,quick detection ,and requires less amount of sample .It is an effective means to screening the tuberculosis specific markers .We found the good diagnosis model through the detection of serum protein by protein fingerprint-ing technology .