CAJ | 학술논문

目的：分离福建省狂犬病毒街毒株，了解病毒株的生物学特性。方法采集疑似狂犬的脑组织，通过细胞培养和乳鼠脑接种分离狂犬病毒，用直接免疫荧光和RT-PCR等方法进行鉴定，并对病毒的分子生物学特性进行研究。结果从疑似狂犬的脑组织中分离出狂犬病毒，TCID50的测定结果显示，细胞毒滴度不高，病毒株对BHK-21细胞的适应性不强，LD50的测定结果显示，病毒株为狂犬病毒强毒株。结论从福建省家犬中成功分离到狂犬病毒街毒株，为狂犬病的实验室研究奠定基础。
목적：분리복건성광견병독가독주，료해병독주적생물학특성。방법채집의사광견적뇌조직，통과세포배양화유서뇌접충분리광견병독，용직접면역형광화RT-PCR등방법진행감정，병대병독적분자생물학특성진행연구。결과종의사광견적뇌조직중분리출광견병독，TCID50적측정결과현시，세포독적도불고，병독주대BHK-21세포적괄응성불강，LD50적측정결과현시，병독주위광견병독강독주。결론종복건성가견중성공분리도광견병독가독주，위광견병적실험실연구전정기출。
In this study ,street trains were isolated from brain of suspected rabid dogs via mouse inoculation technique (MIT) and cell culture inoculation technique (CIT) .Virus was identified by FAT ,RT-PCR and sequencing .Then ,the virus’ biological characteristics were analysed .The TCID50 test results demonstrated that viral titers were not high ,which showed the BHK-21 cells was not the best host cell of rabies viral strain .The LD50 test results showed it was a strong strain of rabies virus .These results laid a strong foundation for rabies laboratory research in Fujian .