中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2014年
7期
669-672
,共4页
余源%陈阳%葛爽%王洋%李巍伟%赵丽娜%刘阿倩%林志强%高雪%田喜凤
餘源%陳暘%葛爽%王洋%李巍偉%趙麗娜%劉阿倩%林誌彊%高雪%田喜鳳
여원%진양%갈상%왕양%리외위%조려나%류아천%림지강%고설%전희봉
C2株蓝氏贾第鞭毛虫%双氢青蒿素%Alpha-7.3 giardin%实时荧光定量RT-PCR
C2株藍氏賈第鞭毛蟲%雙氫青蒿素%Alpha-7.3 giardin%實時熒光定量RT-PCR
C2주람씨가제편모충%쌍경청호소%Alpha-7.3 giardin%실시형광정량RT-PCR
C2 Giardia lamblia%dihydroartemisinin%Alpha-7.3 giardin%real-time reverse transcription PCR
目的：观察双氢青蒿素（dihydroartemisinin ，DHA）对C2株蓝氏贾第鞭毛虫（Giardia lamblia）Alpha-7．3 giar-din （α-贾第素）基因mRNA表达水平的影响，探讨其对蓝氏贾第鞭毛虫骨架蛋白的损伤作用。方法用双氢青蒿素浓度为100μg/mL、200μg/mL的改良TYI-S-33培养基分别培养C2株蓝氏贾第鞭毛虫2 h、4 h、8 h、12 h后，以不含药物组为对照，实时荧光定量RT-PCR检测药物作用后Alpha-7．3 giardin基因mRNA表达水平的变化。结果双氢青蒿素作用虫体后Al-pha-7．3 giardin基因mRNA表达水平明显低于对照组，二者有显著性差异。结论双氢青蒿素对C2株蓝氏贾第鞭毛虫Al-pha-7．3 giardin基因mRNA的表达具有明显的抑制作用，抑制效果与药物浓度和作用时间相关，提示双氢青蒿素对蓝氏贾第鞭毛虫骨架蛋白具有损伤作用。
目的：觀察雙氫青蒿素（dihydroartemisinin ，DHA）對C2株藍氏賈第鞭毛蟲（Giardia lamblia）Alpha-7．3 giar-din （α-賈第素）基因mRNA錶達水平的影響，探討其對藍氏賈第鞭毛蟲骨架蛋白的損傷作用。方法用雙氫青蒿素濃度為100μg/mL、200μg/mL的改良TYI-S-33培養基分彆培養C2株藍氏賈第鞭毛蟲2 h、4 h、8 h、12 h後，以不含藥物組為對照，實時熒光定量RT-PCR檢測藥物作用後Alpha-7．3 giardin基因mRNA錶達水平的變化。結果雙氫青蒿素作用蟲體後Al-pha-7．3 giardin基因mRNA錶達水平明顯低于對照組，二者有顯著性差異。結論雙氫青蒿素對C2株藍氏賈第鞭毛蟲Al-pha-7．3 giardin基因mRNA的錶達具有明顯的抑製作用，抑製效果與藥物濃度和作用時間相關，提示雙氫青蒿素對藍氏賈第鞭毛蟲骨架蛋白具有損傷作用。
목적：관찰쌍경청호소（dihydroartemisinin ，DHA）대C2주람씨가제편모충（Giardia lamblia）Alpha-7．3 giar-din （α-가제소）기인mRNA표체수평적영향，탐토기대람씨가제편모충골가단백적손상작용。방법용쌍경청호소농도위100μg/mL、200μg/mL적개량TYI-S-33배양기분별배양C2주람씨가제편모충2 h、4 h、8 h、12 h후，이불함약물조위대조，실시형광정량RT-PCR검측약물작용후Alpha-7．3 giardin기인mRNA표체수평적변화。결과쌍경청호소작용충체후Al-pha-7．3 giardin기인mRNA표체수평명현저우대조조，이자유현저성차이。결론쌍경청호소대C2주람씨가제편모충Al-pha-7．3 giardin기인mRNA적표체구유명현적억제작용，억제효과여약물농도화작용시간상관，제시쌍경청호소대람씨가제편모충골가단백구유손상작용。
Effects of dihydroartemisinin (DHA) on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lam-blia was investigated in this study to explore the damage to skeleton protein of C 2 Giardia lamblia .Giardia lamblia was culti-vated respectively for 2 ,4 ,8 ,and 12 hours with modified TYI-S-33 medium containing 100 μg/mL and 200 μg/mL DHA , while the control group performed in the same experimental conditions without DHA .The expressive quantity of Alpha-7 .3 gi-ardin mRNA was determined by using real-time reverse transcription PCR ,and then we found that the expressive quantities of Alpha-7 .3 giardin mRNA with DHA were significantly lower than those in the control group .It’s suggested that dihydroarte-misinin has obvious inhibitory effect on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lamblia .The actions of dihydroartemisinin on skeleton protein of C2 Giardia lamblia are effective .
