CAJ | 학술논문

目的：肺炎链球菌可形成生物膜。文中旨在研究青霉素不同最低抑菌浓度（minimal inhibitory concentration， MIC）的肺炎链球菌生物膜形成厚度的差别。方法收集2010年11月至2012年10月南京军区南京总医院、江苏省人民医院、江苏省中医院、南京医科大学第二附属医院、南京市鼓楼医院、东南大学附属中大医院、南京市第一医院等7家医院共147株肺炎链球菌，琼脂稀释法测定青霉素MIC，根据MIC分为≤0．065μg/mL组、0．5μg/mL组、2μg/mL组及≥4μg/mL组，每组选择4珠菌，共16珠菌；96孔板、24孔板培养生物膜24 h，分别在570 nm处测A值并且激光共聚焦扫描显微镜（ confocal laser scanning micro-scope， CLSM）观察生物膜形成。结果生物膜半定量检测及CLSM显示所有菌株都形成生物膜。≤0．065μg/mL组平均A值（0．228±0．063）高于0．5μg/mL组（0．200±0．061）和≥4μg/mL组（0．186±0．050），差异均有统计学意义（ P＜0．05），但与2μg/mL组（0．203±0．049）差异无统计学意义（P＞0．05）；0．5μg/mL组、2μg/mL组≥4μg/mL组A值两两比较的差异无统计学意义（P＞0．05）。结论青霉素MIC高的肺炎链球菌生物膜厚度有减弱的趋势，生物膜的形成是复杂的过程，受多种因素影响。
목적：폐염련구균가형성생물막。문중지재연구청매소불동최저억균농도（minimal inhibitory concentration， MIC）적폐염련구균생물막형성후도적차별。방법수집2010년11월지2012년10월남경군구남경총의원、강소성인민의원、강소성중의원、남경의과대학제이부속의원、남경시고루의원、동남대학부속중대의원、남경시제일의원등7가의원공147주폐염련구균，경지희석법측정청매소MIC，근거MIC분위≤0．065μg/mL조、0．5μg/mL조、2μg/mL조급≥4μg/mL조，매조선택4주균，공16주균；96공판、24공판배양생물막24 h，분별재570 nm처측A치병차격광공취초소묘현미경（ confocal laser scanning micro-scope， CLSM）관찰생물막형성。결과생물막반정량검측급CLSM현시소유균주도형성생물막。≤0．065μg/mL조평균A치（0．228±0．063）고우0．5μg/mL조（0．200±0．061）화≥4μg/mL조（0．186±0．050），차이균유통계학의의（ P＜0．05），단여2μg/mL조（0．203±0．049）차이무통계학의의（P＞0．05）；0．5μg/mL조、2μg/mL조≥4μg/mL조A치량량비교적차이무통계학의의（P＞0．05）。결론청매소MIC고적폐염련구균생물막후도유감약적추세，생물막적형성시복잡적과정，수다충인소영향。
Objective Streptococcus pneumoniae can form biofilms .The aim of this study was to investigate the biofilm forma-tion of Streptococcus pneumoniae and the relationship with antibiotic resistance of penicillin etc . Methods A total of 147 clinical iso-lates of Streptococcus pneumoniae were collected from 7 teaching hospitals in Nanjing from 2010 to 2012.Minimal inhibitory concentration (MIC) of penicillin, erythromycin, cefuroxime and ceftriaxone were determined by agar dilution method .Streptococcus pneumoniae with various penicillin MIC was selected randomly as follow:MIC≤0.065μg/mL, 0.5μg/mL, 2μg/mL and≥4μg/mL, which was incuba-ted to form biofilms in 96-well plates and 24-well plates for 24 hours.The A values at 570 nm was measured and the biofilm was observed through confocal laser scanning microscope ( CLSM) . Results The biofilm semi-quantitative detection and CLSM both displayed that all strains formed biofilms.The A value of the group which penicillin MIC was ≤0.065μg/mL (0.228 ±0.063) was higher than the 0.5μg/mL group (0.200 ±0.061) and the≥4μg/mL group (0.186 ±0.050) (P<0.05) , and there was no difference among the groups which penicillin MIC were 0.5μg/mL, 2μg/mL and≥4μg/mL, respectively (P>0.05).The A value of the group which erythromycin MIC was ≤0.5μg/mL (0.211 ±0.068) was higher than the ≥4μg/mL group (0.201 ±0.052) (P>0.05).The A value of the group sensitive to cefuroxime (0.216 ±0.062) was higher than the group resistant to cefuroxime (0.196 ±0.054) (P<0.05). Conclusion Streptococcus pneumoniae can form biofilms .Streptococcus pneumoniae with high antibiotics MIC has a trend of weakened biofilm formation .