CAJ | 학술논문

目的： Morris水迷宫实验是一种评价空间学习记忆能力的经典方法，是评价痴呆动物模型复制结果的客观指标。近年来痴呆动物模型已成为研究老年性痴呆的重要手段。文中评价3种常见拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异。方法分别使用SD大鼠、昆明种小鼠以及快速老化小鼠制备Aβ脑室注射致痴呆模型、东莨菪碱腹腔注射致记忆障碍模型、快速老化模型。采用Morris水迷宫行为学测试方法，将这3种模型通过与其相应的对照组比较，考察其行为学差异。结果东莨菪碱模型与快速老化模型逃避潜伏期时间短于相应对照组，其中以东莨菪碱模型差异尤为显著[（35．47±3．78）s vs （50．61±3．94）s， P＜0．01]；快速老化组模型与对照组差异有统计学意义[（17．39±4．71）s vs （33．79±7．81）s， P＜0．05]；Aβ致痴呆模型与其对照组比较，模型定位航行实验成绩差异无统计学意义（P＞0．05）。结论东莨菪碱腹腔注射致记忆障碍模型较其他2种常用模型更为理想。 Morris水迷宫行为学测试方法是一种评价拟痴呆动物的理想方法。
목적： Morris수미궁실험시일충평개공간학습기억능력적경전방법，시평개치태동물모형복제결과적객관지표。근년래치태동물모형이성위연구노년성치태적중요수단。문중평개3충상견의치태동물모형재Morris수미궁행위학측시중학습기억행위적차이。방법분별사용SD대서、곤명충소서이급쾌속노화소서제비Aβ뇌실주사치치태모형、동랑탕감복강주사치기억장애모형、쾌속노화모형。채용Morris수미궁행위학측시방법，장저3충모형통과여기상응적대조조비교，고찰기행위학차이。결과동랑탕감모형여쾌속노화모형도피잠복기시간단우상응대조조，기중이동랑탕감모형차이우위현저[（35．47±3．78）s vs （50．61±3．94）s， P＜0．01]；쾌속노화조모형여대조조차이유통계학의의[（17．39±4．71）s vs （33．79±7．81）s， P＜0．05]；Aβ치치태모형여기대조조비교，모형정위항행실험성적차이무통계학의의（P＞0．05）。결론동랑탕감복강주사치기억장애모형교기타2충상용모형경위이상。 Morris수미궁행위학측시방법시일충평개의치태동물적이상방법。
Objective The study was to evaluate the behavioral differences in learning and memory abilities among three ani -mal models of Alzheimer′s disease. Methods Three SD rat models(n=20) were used in this study.The first SD rat model was es-tablished by intracerebral injection of Aβ25-35 into the bilateral hippocampus , the second mouse model was induced by intraperitoneal in-jection of scopolamine and the third was a senescence accelerated mouse model .Morris Water maze test was performed to investigate behavioral differences by comparison to corresponding blank control groups ( sham operation group , blank group and P8 group ) . Results The learning and memory abilities of senescence accelerated rats and scopolamine -treated rats were worse than those of the corresponding control groups, especially the scopolamine-treated rat ([35.47 ±3.78]s vs [50.61 ±3.94]s, P<0.01).There was no significant difference between Aβrats and sham-operated rats(P>0.05). Conclusion The model of Alzheimer′s disease in-duced by intraperitoneal injection of scopolamine represents more distinct changes in learning and memory abilities .Morris water maze test can be used to well evaluate whether the scopolamine-induced model is successfully established or not .