CAJ | 학술논문

目的：探讨天麻素对神经病理性疼痛大鼠的镇痛作用及其机制，为临床开发新的镇痛药物奠定基础。方法SD大鼠随机分为坐骨神经慢性压迫性损伤组(n=32)和假手术组(n=8)，自术后第8天分别腹腔注射天麻素治疗，用电子Von-Frey测痛仪测定大鼠机械缩足反射阈值，热痛刺激仪测定大鼠热痛缩足反应潜伏期。取脊髓腰膨大及L4/L6背根神经节，采用Westem blotting法测定脊髓背角和背根神经节p-ERK1/2的表达。结果与假手术组相比，对照组和天麻素治疗组各时点机械缩足反射阈值降低、热痛缩足反应潜伏期缩短，脊髓背角及背根神经节p-ERK1/2均出现表达上调(P<0.05)；与对照组相比，天麻素治疗组在给药后7d机械缩足反射阈值回升、热痛缩足反应潜伏期延长；在给药14d后脊髓背角及背根神经节p-ERK1/2表达下调(P<0.05)。结论天麻素可减轻大鼠神经病理性痛，其机制可能与抑制脊髓背角和背根神经节p-ERK1/2通路激活有关。
목적：탐토천마소대신경병이성동통대서적진통작용급기궤제，위림상개발신적진통약물전정기출。방법SD대서수궤분위좌골신경만성압박성손상조(n=32)화가수술조(n=8)，자술후제8천분별복강주사천마소치료，용전자Von-Frey측통의측정대서궤계축족반사역치，열통자격의측정대서열통축족반응잠복기。취척수요팽대급L4/L6배근신경절，채용Westem blotting법측정척수배각화배근신경절p-ERK1/2적표체。결과여가수술조상비，대조조화천마소치료조각시점궤계축족반사역치강저、열통축족반응잠복기축단，척수배각급배근신경절p-ERK1/2균출현표체상조(P<0.05)；여대조조상비，천마소치료조재급약후7d궤계축족반사역치회승、열통축족반응잠복기연장；재급약14d후척수배각급배근신경절p-ERK1/2표체하조(P<0.05)。결론천마소가감경대서신경병이성통，기궤제가능여억제척수배각화배근신경절p-ERK1/2통로격활유관。
Objective To investigate the analgesic effects and mechanism of gastrodin on the neuropathic pain in rats, and screen novel analgesics for clinical treatment of neuropathic pain diseases. Methods Forty male SD rats were randomly divided into 5 groups as sham operation group, control group, gastrodin 30 mg/kg group, gastrodin 60 mg/kg group, gastrodin120 mg/kg group. From the 8th day post-ligation, different doses of gastrodin were injected into abdominal cavity and the pain thresholds before and after intraperitoneal injection of the different doses of gastrodin were measured by the mechanical and hot stimulus. The expressions of p-ERK1/2 in L4/6 dorsal root ganglion neurons and dorsal horn was measured by the methods of Western blotting. Results Compared with sham operation group, the rats in CCI group developed a decrease in mechanical withdrawal threshold and thermal withdrawal latency associated with an increase in the activity of p-ERK1/2 in dorsal horn and dorsal root ganglion. Compared with CCI group, 7 day treatment with gastrodin significantly attenuated mechanical allodynia and thermal hyperalgesia, and these effects were correlated with inhibiting the hyper-activation of p-ERK1/2 after 14 days treatment with gastrodin. Conclusions Gastrodin has beneficial effects on neuropathic pain and the analgesic effects of gastrodin would be mediated by the ERK1/2 signaling pathways.