临床医学工程
臨床醫學工程
림상의학공정
CLINICAL MEDICAL ENGINEERING
2014年
7期
829-831
,共3页
江勇%郭卉欣%赖赛麟%钱明%李海成%黎贞燕%余丽%江振友%周琳%钟球%陈涛
江勇%郭卉訢%賴賽麟%錢明%李海成%黎貞燕%餘麗%江振友%週琳%鐘毬%陳濤
강용%곽훼흔%뢰새린%전명%리해성%려정연%여려%강진우%주림%종구%진도
巨噬细胞%IFN-γ%TNF-α%活化
巨噬細胞%IFN-γ%TNF-α%活化
거서세포%IFN-γ%TNF-α%활화
Macrophages%IFN-γ,TNF-α%Activation
目的：摸索利用IFN-γ诱导THP-14巨噬细胞活化的方法，筛选用于检测巨噬细胞活化状态的最佳细胞因子和最佳检测时间。方法PMA(佛波酯)诱导THP-1分化成为巨噬细胞，两次加入IFN-γ诱导巨噬细胞活化(两步法)，ELISA法检测不同浓度和不同刺激时间培养上清中细胞因子TNF-α、IL-10、IL-12、IL-23的水平。结果IFN-γ诱导的巨噬细胞可大量分泌TNF-α，分泌量大于1000灶早/皂L，而IL-10、IL-12、IL-23分泌量少或未见明显分泌；两次IFN-γ诱导巨噬细胞活化的最低浓度均为2.5ng/mL，初次最佳刺激时间为8~16h，再次最佳刺激6~18h。结论IFN-γ可诱导巨噬细胞活化，两次IFN-γ刺激的效果比单次刺激效果好，TNF-α可作为巨噬细胞活化的理想检测指标。
目的：摸索利用IFN-γ誘導THP-14巨噬細胞活化的方法，篩選用于檢測巨噬細胞活化狀態的最佳細胞因子和最佳檢測時間。方法PMA(彿波酯)誘導THP-1分化成為巨噬細胞，兩次加入IFN-γ誘導巨噬細胞活化(兩步法)，ELISA法檢測不同濃度和不同刺激時間培養上清中細胞因子TNF-α、IL-10、IL-12、IL-23的水平。結果IFN-γ誘導的巨噬細胞可大量分泌TNF-α，分泌量大于1000竈早/皂L，而IL-10、IL-12、IL-23分泌量少或未見明顯分泌；兩次IFN-γ誘導巨噬細胞活化的最低濃度均為2.5ng/mL，初次最佳刺激時間為8~16h，再次最佳刺激6~18h。結論IFN-γ可誘導巨噬細胞活化，兩次IFN-γ刺激的效果比單次刺激效果好，TNF-α可作為巨噬細胞活化的理想檢測指標。
목적：모색이용IFN-γ유도THP-14거서세포활화적방법，사선용우검측거서세포활화상태적최가세포인자화최가검측시간。방법PMA(불파지)유도THP-1분화성위거서세포，량차가입IFN-γ유도거서세포활화(량보법)，ELISA법검측불동농도화불동자격시간배양상청중세포인자TNF-α、IL-10、IL-12、IL-23적수평。결과IFN-γ유도적거서세포가대량분비TNF-α，분비량대우1000조조/조L，이IL-10、IL-12、IL-23분비량소혹미견명현분비；량차IFN-γ유도거서세포활화적최저농도균위2.5ng/mL，초차최가자격시간위8~16h，재차최가자격6~18h。결론IFN-γ가유도거서세포활화，량차IFN-γ자격적효과비단차자격효과호，TNF-α가작위거서세포활화적이상검측지표。
Objective To find out the optimal cytokines and detection time when the activation state of macrophages derived from THP-1 induced by IFN-γ was detected. Methods THP-1 cells which differentiated into macrophages induced by phorbol ester (PMA), IFN-γwere added to induce the activation of macrophages for twice. ELISA was used to detect the concentration of TNF-α, IL-10, IL-12 and IL-23 in cultured supernatant in different stimulation concentration and stimulation time. Results The macrophages induced by IFN-γcould secrete a mass of TNF-αwhich could increase to over 1 000 ng/mL. At the same time, there were no obvious concentration changes of IL-10, IL-12 and IL-23. The minimum concentration of IFN-γto induce macrophages activation was 2.5 ng/mL for each time. The optimal first stimulation time was 8 to 16 hours while the optimal second stimulation time was 6 to 18 hours. Conclusions Macrophages can be induced by IFN-γ. Stimulation by IFN-γfor twice is more effective than once, therefore, it can be an ideal detection index for macrophage activation.
