CAJ | 학술논문

目的：对酶联免疫吸附法(ELISA)测定牛奶中链霉素残留量的测量不确定度进行评定。方法分析了该方法的不确定度来源，建立数学模型，并进行分析后找到主要影响因素。结果当牛奶中链霉素含量为7.7 ng/mL时，其扩展不确定度为1.52 ng/mL (k=2)。其中，测量样品重复性、反应温度及回收率影响较大。结论采用酶联免疫吸附法(ELISA)测定牛奶中链霉素含量应保证实验具有良好的重复性，严格控制实验反应条件，并且保证样品回收率符合要求。
목적：대매련면역흡부법(ELISA)측정우내중련매소잔류량적측량불학정도진행평정。방법분석료해방법적불학정도래원，건립수학모형，병진행분석후조도주요영향인소。결과당우내중련매소함량위7.7 ng/mL시，기확전불학정도위1.52 ng/mL (k=2)。기중，측량양품중복성、반응온도급회수솔영향교대。결론채용매련면역흡부법(ELISA)측정우내중련매소함량응보증실험구유량호적중복성，엄격공제실험반응조건，병차보증양품회수솔부합요구。
Objective The uncertainty in detecting streptomycin in milk by ELISA was evaluated. Methods The source of uncertainty of the whole determination procedure was analyzed, and the mathe-matical model was developed. Results The main influencing factors and the combined uncertainty were obtained. The expanded uncertainty was 1.52 ng/mL (k=2) with 7.7 ng/mL of the content of streptomycin. The major sources of uncertainty of measurement were the measuring repeatability and the reaction temper-ature, and the sample recovery rate. Conclusion It is necessary to control repeatability of measurement and experimental reaction conditions to make sure the testing results are accurate by ELISA. It also should en-sure that the sample recovery rate could meet the requirements.