CAJ | 학술논문

目的：改进国标法检测乳及乳制品中亚硝酸盐、硝酸盐的样品前处理方法。方法样品经乙腈沉淀脂肪蛋白质后，采用相应的方法提取和净化，以氢氧化钾溶液为淋洗液，阴离子交换柱分离，电导检测器检测。以保留时间定性，外标法定量。结果亚硝酸盐回收率80%～110%，硝酸盐回收率90%～110%，方法精密度在10%之内。结论本方法可提高检测准确性，缩小检测偏差，适合于乳及乳制品定量测定。
목적：개진국표법검측유급유제품중아초산염、초산염적양품전처리방법。방법양품경을정침정지방단백질후，채용상응적방법제취화정화，이경양화갑용액위림세액，음리자교환주분리，전도검측기검측。이보류시간정성，외표법정량。결과아초산염회수솔80%～110%，초산염회수솔90%～110%，방법정밀도재10%지내。결론본방법가제고검측준학성，축소검측편차，괄합우유급유제품정량측정。
Objective To improve the pretreatment method of national standard to detect nitrate and nitrite in Milk and dairy products. Methods Samples were extracted by acetonitrile, and after protein and fat precipitation, the extract was purified by anion exchange column, potassium hydroxide solution as leached solution. Nitrate and nitrite were detected by conductivity detector. Results The recoveries of ni-trate and nitrite were 80%~110%and 90%~110%respectively, and the precision of the method were within 10%. Conclusion This method can improve the detection accuracy and reduce the detection error, and is suitable for the quantitative detection of nitrate and nitrite in milk and dairy products.