CAJ | 학술논문

目的：建立一种采用柱前衍生-反相高效液相色谱法检测杨树花中游离氨基酸和水解氨基酸含量的方法。方法以异硫氰酸苯酯进行柱前衍生,采用高效液相色谱法检测杨树花中游离氨基酸和水解氨基酸含量。结果21种氨基酸线性范围为0.0019~2.7μmol·mL-1, R2均＞0.9990；21种游离氨基酸的平均加样回收率在88.25%~100.04%之间、RSD 为0.57%~2.73%(n=6),水解氨基酸的平均加样回收率在90.21%~100.01%之间、RSD 为0.52%~2.32%(n=6)。结论该方法稳定、快速、精密度高、线性范围宽,适合杨树花中游离氨基酸和水解氨基酸的含量测定。
목적：건립일충채용주전연생-반상고효액상색보법검측양수화중유리안기산화수해안기산함량적방법。방법이이류청산분지진행주전연생,채용고효액상색보법검측양수화중유리안기산화수해안기산함량。결과21충안기산선성범위위0.0019~2.7μmol·mL-1, R2균＞0.9990；21충유리안기산적평균가양회수솔재88.25%~100.04%지간、RSD 위0.57%~2.73%(n=6),수해안기산적평균가양회수솔재90.21%~100.01%지간、RSD 위0.52%~2.32%(n=6)。결론해방법은정、쾌속、정밀도고、선성범위관,괄합양수화중유리안기산화수해안기산적함량측정。
Objective To determine the contents of 21 free and hydrolyzed amino acids from Flos Po-puli Tomentosae by pre-column derivatization reversed phase high performance liquid chromatography (RP-HPLC). Methods The samples were derived with phenylisothiocyanate (PITC) and HPLC method was adopted. Gradient elution chromatography was applied to determine free and hydrolyzed amino acids con-tents in Flos Populi Tomentosae. Results The 21 amino acids showed a good linearity in the ranges of 0.0019～2.7 μmol·mL-1 with the correlation coefficients over 0.9990. The average recoveries of free amino acids were 88.25%～100.04%with relative standard deviation of 0.57%～2.73%(n=6);and the average reco-veries of total amino acids were 90.21%～100.01% with relative standard deviation of 0.52%～2.32%(n=6). Conclusion The method is sensitive, accurate and reproducible, and it is suitable for the determination of amino acids in many other samples.