食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
7期
2138-2145
,共8页
免疫层析试纸条%酶%胶体金%胶体碳%有机荧光分子%镧系元素离子%量子点%电化学活性探针
免疫層析試紙條%酶%膠體金%膠體碳%有機熒光分子%鑭繫元素離子%量子點%電化學活性探針
면역층석시지조%매%효체금%효체탄%유궤형광분자%란계원소리자%양자점%전화학활성탐침
immunochromatographic test strip%enzyme%colloidal gold%carbon nanoparticle%organic fluo-rophore%lanthanide-based chelate%quantum dot%electrochemically active reporter
免疫层析试纸条(immunochromatographic test strips, ICTSs)有效结合了层析法卓越的分离能力和常规免疫分析技术的高度特异性,加之其便携性,该技术无疑为灵敏、定量的现场检测提供了一个理想的平台。近年来随着检测方法和技术的不断革新,包括生色、发光及电化学信号产生型等在内的多种探针不断涌现,它们或直接共价结合靶标蛋白或装载于纳米颗粒中用于免疫层析试纸条的标记。本文旨在总结归纳当前 ICTSs 标记探针的最新进展,详尽解析各类探针性质、发展、优劣势及其在生物分析中的应用,以期为研究者在设计试纸条时适宜探针的选择提供有力的技术支持。
免疫層析試紙條(immunochromatographic test strips, ICTSs)有效結閤瞭層析法卓越的分離能力和常規免疫分析技術的高度特異性,加之其便攜性,該技術無疑為靈敏、定量的現場檢測提供瞭一箇理想的平檯。近年來隨著檢測方法和技術的不斷革新,包括生色、髮光及電化學信號產生型等在內的多種探針不斷湧現,它們或直接共價結閤靶標蛋白或裝載于納米顆粒中用于免疫層析試紙條的標記。本文旨在總結歸納噹前 ICTSs 標記探針的最新進展,詳儘解析各類探針性質、髮展、優劣勢及其在生物分析中的應用,以期為研究者在設計試紙條時適宜探針的選擇提供有力的技術支持。
면역층석시지조(immunochromatographic test strips, ICTSs)유효결합료층석법탁월적분리능력화상규면역분석기술적고도특이성,가지기편휴성,해기술무의위령민、정량적현장검측제공료일개이상적평태。근년래수착검측방법화기술적불단혁신,포괄생색、발광급전화학신호산생형등재내적다충탐침불단용현,타문혹직접공개결합파표단백혹장재우납미과립중용우면역층석시지조적표기。본문지재총결귀납당전 ICTSs 표기탐침적최신진전,상진해석각류탐침성질、발전、우열세급기재생물분석중적응용,이기위연구자재설계시지조시괄의탐침적선택제공유력적기술지지。
Immunochromatographic test strips (ICTSs) which combine the excellent separation ability of chro-matography with the high specificity and sensitivity of conventional immunoassay, has served as an ideal platform for quantitative and sensitive on-site sensing. With the advances in detection methodology and instrumentation, ver-satile labels, colorimetric, fluorescent and electrochemical signals-developing, directly conjugated or loaded on na-nocarriers, have been developed for ICTSs. This paper was aimed to summarize the recent advances in the reporters developed for ICTSs, with their properties, development as well as individual strength and weakness detailedly illu-strated so as to facilitate our screening of suitable ones while designing ICTSs for specific aims.
