CAJ | 학술논문

目的：评估食品中单增李斯特氏菌SureTect实时PCR检测方法的性能。方法按照ISO 16140：2003/Amd 1：2011《食品和动物饲料微生物学-可替代方法的验证方案》的要求对该方法进行了实验室内的方法比较研究(即实验室内的验证)。结果对于不同的食品类别，该方法的相对准确性在77%~85%之间，相对特异性在72%~83%之间，相对灵敏度在72%~91%之间，相对检测限在0.02~0.06 CFU/g。统计检验表明，该方法与参照方法在相对准确性、相对特异性和相对灵敏度方面无显著性差异，两种方法的相对检测限也无显著性差异。使用50株目标菌株和30株非目标菌株进行方法选择性实验，两种方法检测结果均一致。结论单增李斯特氏菌SureTect实时PCR检测方法是一种快速、准确和灵敏的检测方法。
목적：평고식품중단증리사특씨균SureTect실시PCR검측방법적성능。방법안조ISO 16140：2003/Amd 1：2011《식품화동물사료미생물학-가체대방법적험증방안》적요구대해방법진행료실험실내적방법비교연구(즉실험실내적험증)。결과대우불동적식품유별，해방법적상대준학성재77%~85%지간，상대특이성재72%~83%지간，상대령민도재72%~91%지간，상대검측한재0.02~0.06 CFU/g。통계검험표명，해방법여삼조방법재상대준학성、상대특이성화상대령민도방면무현저성차이，량충방법적상대검측한야무현저성차이。사용50주목표균주화30주비목표균주진행방법선택성실험，량충방법검측결과균일치。결론단증리사특씨균SureTect실시PCR검측방법시일충쾌속、준학화령민적검측방법。
Objective In order to validate the SureTect real-time PCR test for Listeria monocytogenes in food. Methods Comparison study was conducted between the method and the reference method detailed in National Standard GB 4789.30 Microbiological examination of food Hygiene-Examination of Listeria mono-cytogenes according to ISO 16140:2003/Amd:2011 Microbiology of food and animal feeding stuffs-protocol for the validation of alternative method. Results For different food categories, the relative accuracy of the method was between 77%and 85%, the relative specificity was between 72%and 83%, and the relative sensi-tivity was between 72%and 91%. The relative detection level was between 0.02 CFU/g and 0.06 CFU/g. Sta-tistical tests proved that there were no significant difference between the relative accuracy of two methods for relative specificity, relative sensitivity and relative detection level. As to the selectivity, fifty target strains and thirty non-target strains were tested by the two methods. The results of the strains were congruent by the Sure-Tect real-time PCR test. Conclusion Results proved that the SureTect Listeria monocytogenes assay is a rapid, accurate, and sensitive detection method.