CAJ | 학술논문

目的：建立一种新型基于多肽标准品体系的人乳α-乳白蛋白的定量检测方法。方法从人乳α-乳白蛋白筛选出特异性多肽,并建立对应的检测方法；利用化学方法人工合成特异性多肽、同位素标记特异性多肽和内标。结果本方法所选择的多肽具有高度特异性,α-乳白蛋白的检测灵敏度为8.0 mg/100 g,精密度均小于5.22%,回收率均在97.21%~102.45%之间。结论本方法建立了利用特异性多肽检测人α-乳白蛋白的方法,具有较高的准确度、灵敏度和稳定性。通过对447份人乳样品进行检测,初步探索了人乳中α-乳白蛋白的含量范围,为人乳化配方奶粉的华人配方奠定理论基础。
목적：건립일충신형기우다태표준품체계적인유α-유백단백적정량검측방법。방법종인유α-유백단백사선출특이성다태,병건립대응적검측방법；이용화학방법인공합성특이성다태、동위소표기특이성다태화내표。결과본방법소선택적다태구유고도특이성,α-유백단백적검측령민도위8.0 mg/100 g,정밀도균소우5.22%,회수솔균재97.21%~102.45%지간。결론본방법건립료이용특이성다태검측인α-유백단백적방법,구유교고적준학도、령민도화은정성。통과대447빈인유양품진행검측,초보탐색료인유중α-유백단백적함량범위,위인유화배방내분적화인배방전정이론기출。
Objective To establish a quantitative detection ofα-lactalbumin in human milk with peptide standard system. Methods The signature peptide forα-lactalbumin was searched and selected from the pro-tein digestion products. The signature peptide, isotope-labelled signature peptide and internal standard peptide were also synthesized and used for establishing the quantification method. Results The limit of quantification for α-lactalbumin was 8.0 mg/100 g. Reproducibility were below 5.22% and spiked recoveries were between 97.21%~102.45%. Conclusion The validation showed that the method was with high selectivity, accuracy, reproducibility and sensitivity. The analysis results of 447 human milk samples provided the theoretical basis for infant formula suitable for Chinese babies.