CAJ | 학술논문

目的：应用高压液相色谱法同时测定人乳中视黄醇、α-生育酚、叶黄素和β-胡萝卜素。方法人乳样品经皂化后，石油醚乙醚提取，氮吹浓缩后，用 C30柱分离，经紫外检测器和荧光检测器检测4种被测物。结果4种脂溶性维生素在各自浓度范围内线性良好，相关系数均大于0.9990；三浓度水平加标回收率在70%~120%之间，相对标准偏差均小于20%。结论本文将校准后的标准品用液相色谱法进行纯度鉴定，提高了检测的准确度。通过方法学验证了本方法的准确性，灵敏性和稳定性，可同时测定人乳中视黄醇，α-生育酚，叶黄素和β-胡萝卜素的含量。通过对100个成熟乳样品的分析，初步建立了人乳中脂溶性维生素含量数据库。
목적：응용고압액상색보법동시측정인유중시황순、α-생육분、협황소화β-호라복소。방법인유양품경조화후，석유미을미제취，담취농축후，용 C30주분리，경자외검측기화형광검측기검측4충피측물。결과4충지용성유생소재각자농도범위내선성량호，상관계수균대우0.9990；삼농도수평가표회수솔재70%~120%지간，상대표준편차균소우20%。결론본문장교준후적표준품용액상색보법진행순도감정，제고료검측적준학도。통과방법학험증료본방법적준학성，령민성화은정성，가동시측정인유중시황순，α-생육분，협황소화β-호라복소적함량。통과대100개성숙유양품적분석，초보건립료인유중지용성유생소함량수거고。
Objective To establish a rapid method for simultaneous determination of retinoid,α-tocopherol, lutein andβ-carotene in human milk by high performance liquid chromatography. Methods The human milk sample was saponified and extracted with a mixture of petroleum ether and diethyl ether. The sample was separated after concentration by a C30 column and detected by the UV detector and FLD detector. Results The method showed a good linearity over with R2>0.9990. The recoveries at three spiked levels were between 70%~120%. The relative standard deviation was less than 20%. Conclusion The purity of standard was analyzed using HPLC after standardization with UV spectrometer, thus the accuracy of the method was improved. The method validation showed a high accuracy, sensitivity and stability. This method could be used to simultaneous determination of retinoid, α-tocopherol, lutein and β-carotene in human milk. A preliminary database of the fat-soluble vitamin concentration in human milk was established with 100 mature human milk samples.