CAJ | 학술논문

目的研究姜黄素微乳对白血病K562细胞增殖与凋亡的影响.方法制备姜黄素微乳,用四甲基偶氮唑蓝(MTT)方法观察其对K562细胞增殖作用的影响.用AnnexinV-PI双染、流式细胞术分析检测姜黄素微乳对K562细胞早期凋亡率的影响；用反转录PCR检测姜黄素微乳对K562细胞WT1基因表达的影响.均与姜黄素溶液处理的细胞进行比较.结果制备的姜黄素微乳澄清透明,特性稳定,能剂量依赖性抑制K562细胞的增殖率,2.5、5、10 μg/ml姜黄素微乳作用24 h,细胞增殖抑制率为24％、46％、68％,而同等浓度姜黄素溶液处理后细胞增殖抑制率分别为6％、14％、25％.姜黄素微乳促凋亡作用也优于姜黄素溶液.姜黄素微乳抑制K562细胞WT1 mRNA表达的作用(0.190±0.036)也较姜黄素溶液(0.456±0.047)强.结论以微乳为载体的姜黄素性状稳定.姜黄素微乳在抑制K562细胞增殖、促进凋亡及抑制WT1基因表达方面均显著强于普通姜黄素溶液.为临床应用姜黄素微乳治疗肿瘤提供了有力的理论依据.
목적 연구강황소미유대백혈병K562세포증식여조망적영향.방법 제비강황소미유,용사갑기우담서람(MTT)방법관찰기대K562세포증식작용적영향.용AnnexinV-PI쌍염、류식세포술분석검측강황소미유대K562세포조기조망솔적영향；용반전록PCR검측강황소미유대K562세포WT1기인표체적영향.균여강황소용액처리적세포진행비교.결과 제비적강황소미유징청투명,특성은정,능제량의뢰성억제K562세포적증식솔,2.5、5、10 μg/ml강황소미유작용24 h,세포증식억제솔위24％、46％、68％,이동등농도강황소용액처리후세포증식억제솔분별위6％、14％、25％.강황소미유촉조망작용야우우강황소용액.강황소미유억제K562세포WT1 mRNA표체적작용(0.190±0.036)야교강황소용액(0.456±0.047)강.결론 이미유위재체적강황소성상은정.강황소미유재억제K562세포증식、촉진조망급억제WT1기인표체방면균현저강우보통강황소용액.위림상응용강황소미유치료종류제공료유력적이론의거.
Objective To determine the effects of curcumin microemulsion on proliferation and apoptosis of the human leukemia cell line K562 cells.Methods The curcumin microemulsion was prepared with the routine procedure.MTT assay was used to determine cell proliferation in cultured K562 cells,flow cytometry analysis was applied to examine cell apoptosis,and WT1 mRNA was determined with RT-PCR.The results about curcumin microemulsion were compared with these on curcumin.Results The prepared curcumin microemulsion was a stable clear solution with diameter of 10-100 nm.Curcumin microemulsion inhibited K562 cell proliferation by 24％,46％,68％with a 24 h incubation at dose of 2.5 μg/ml,5.0 μg/ml,and 10.0 μg/ml respectively,whereas curcumin reduced the proliferation by 6％,14％,25％at equal concentrations.WT1 mRNA level of curcumin microemulsion group(0.190±0.036)was reduced stronger than that of curcusin group(0.456±0.047).Conclusions Microemulsion is a great carrier for curcumin.Curcumin microemulsion is more effective in inhibiting proliferation,pro-apoptosis,and reducing WT1 gene expression than curcumin.A strong basis of medical value for the use of curcumin microemulsion to treat tumors is provided.