CAJ | 학술논문

目的探讨葛根总黄酮(PRF)体外诱导人类急性单核细胞白血病SHI-1细胞发生部分分化的可能作用.方法以不同浓度PRF作用SHI-1细胞不同时间,四甲基偶氮唑蓝比色法检测细胞增殖抑制率,流式细胞术(FCM)检测细胞周期,硝基四唑氮蓝(NBT)实验检测细胞还原率,FCM检测细胞表面分化抗原CD11b及CD14的表达.结果 10 ～ 50 μg/ml PRF呈时间-剂量依赖性抑制SHI-1细胞增殖,使细胞阻滞于S期.以10、30、50 μg/ml的PRF药物分别处理SHI-1细胞48 h,SHI-1细胞的NBT还原率随着药物浓度的升高而逐渐增高(P＜0.05),SHI-1细胞表面的分化抗原CD14也同样随着药物浓度的升高而表达增高.结论 10、30、50μg/ml的PRF可体外诱导SHI-1细胞发生部分分化.
목적 탐토갈근총황동(PRF)체외유도인류급성단핵세포백혈병SHI-1세포발생부분분화적가능작용.방법 이불동농도PRF작용SHI-1세포불동시간,사갑기우담서람비색법검측세포증식억제솔,류식세포술(FCM)검측세포주기,초기사서담람(NBT)실험검측세포환원솔,FCM검측세포표면분화항원CD11b급CD14적표체.결과 10 ～ 50 μg/ml PRF정시간-제량의뢰성억제SHI-1세포증식,사세포조체우S기.이10、30、50 μg/ml적PRF약물분별처리SHI-1세포48 h,SHI-1세포적NBT환원솔수착약물농도적승고이축점증고(P＜0.05),SHI-1세포표면적분화항원CD14야동양수착약물농도적승고이표체증고.결론 10、30、50μg/ml적PRF가체외유도SHI-1세포발생부분분화.
Objective To explore the possible effects on differentiation of SHI-1 cells induced by puerariae radix flavones(PRF)in vitro.Methods SHI-1 cells were treated with PRF in various concertration,then the inhibitory effects of cell proliferation were detected by MTT assay,the cell cycles were analyzed by flow cytometry(FCM),the cells reduction rates were detected by NBT reduction test,and the expression of CD11b and CD14 were tested by FCM.Results 10-50 μg/ml PRF could inhibit the proliferation of SHI-1 cells in a time-and dose-dependent manner,and the cell cycles were arrested in S phase.When SHI-1 cells were treated with 10,30 and 50 μg/ml PRF in 48 houres respectively,the NBT reduction rates of cells were increased in a dose-dependent with PRF(P＜0.05),and the expression of cells surface differentiation antigen CD14 was also increased along with the concentration of PRF.Conclusion The SHI-1 cells could be induced to differentiation partially after treated with 10,30 and 50 μg/ml PRF in vitro.